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Description
Human HDAC6 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes and remove the supernatant for analysis. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, but avoid repeated freeze-thaw cycles. Preparation for Assay: 1. Remove the reagent kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. To prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a histone deacetylase 6 (HDAC6) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of histone deacetylase 6 (HDAC6) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Histone Deacetylase 6 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Histone deacetylase 6 is an enzyme encoded by the HDAC6 gene. Disorders associated with HDAC6 include chondrodysplasia with lamellar processes, distinctive humeral processes, hydrocephalus, and microphthalmia and humeral processes. Pathways associated with HDAC6 include activated PKN1, which stimulates transcription of the AR (androgen receptor)-regulated genes KLK2 and KLK3, and the BRCA1 pathway. Gene ontology annotations associated with this gene include enzyme binding and ubiquitin-protein ligase binding. An important homolog of this gene is HDAC10. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenate, cell culture supernatant |
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4.5 ★★★★★
Based on 19 reviews
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Product Reviews
★★★★★ 5
Works great
Size: 6ft, Color: Silver
Just what I needed for my Dept 56 Christmas display. In past years, it's been a challenge to find enough open plugs for my display. This power strip completely solved that problem. I wish I had found this sooner.
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Reviewed in the United States on April 23, 2026
★★★★★ 4
Better than expected - big office cleanup
Size: 6ft, Color: Yellow, Size: 6ft, Color: Yellow
I got this power strip because it seemed like the most plausible one available, notwithstanding the reviews which particularly complained about it being prone to overheating. I certainly didn't want to burn down my home, so I tested it pretty thoroughly before I got anywhere near installing it.
The power strip arrived exactly as claimed. It is indeed 45 inches long with sixteen outlets. The power cord is substantial-feeling, and feels like it's probably 14 AWG, or possibly #12 (Pic 1), and was the length I expected (6' in my case). The mounting brackets are sufficient to mount the power strip, but only at the ends, and they can't be concealed. This means that things don't end up as solid as one would like, and if you really care about aesthetics you'll end up being offended, but hey this is a power strip, and if you didn't have it the power cord mess would be way uglier.
The outlets themselves are spaced out well enough that there's enough room to put in fairly wide wall wart transformers, but not those which have the plug pins configured such that the transformer has to point sideways. This means that if you want to be able to fill all of the slots, you can only put one of these in, and it has to go at the right-hand end. If you locate it on the left then it covers the switch. In my case, I filled the strip up completely, with one sideways transformer at the right hand end, and I still have many more plugs which won't go, but I've at least managed to improve my cable management a fair bit.
Before I installed it, I tested it by boiling an electric kettle using the outlet which is the farthest from the switch (Pic 2). This is the most stressful way to test things, since is stresses all of the wiring and intermediate outlets before it even gets to the one I was using. The kettle draws 11.6 Amps, so about 1400W (though it's amps that actually matter). I then watched the thermal behaviour of the power strip as it worked. As can be seen the first thermograph, Pic 3, the power strip itself stayed cool, indeed barely above ambient. The power meter and power cord going to the kettle are on the right. Pic 4 shows a detail of the switch end of the power strip. The switch never heated to above 77F, which was again barely above ambient. Pic 5 shows a detail of the power meter shown in Pic 2. As can be easily seen, the power cord for the kettle warms up a bit, reaching 100F, but this is clearly the kettle's power cord and plug warming up, not the power strip itself. Pic 6 shows a detail of the unused outlets on the strip. These all warmed up a little, but never got over 83F. To me, this is negligible.
As a result of this testing, I had no problems with going ahead and installing the power strip. I knew I'd be filling the power strip, but just adding up the devices I planned to plug in (two desktops, four screens, and numerous other devices), I knew that I wouldn't need more than about 4.5 amps, so I have at least 2.6X headroom. Pic 7 shows a part of the power strip as installed - I couldn't get far enough back to show all of it. Pic 8 is a thermograph of the same, with the power strip running across the middle of the picture. The how spots on the power strip are all transformers - clearly not very efficient, but not actually more than warm, and not the power strip's fault. By contrast, the really hot items visible are a DSL router on the far right, and a VOIP phone device lower on the right. Thus, I conclude that there's nothing untoward with the power strip. Lots of the devices I use are inefficient and/or run fairly hot, but if my house burns down, it doesn't look like it's going to be because of this power strip.
All told, this product seems to be very good value for something that actually cleans up my life and has this many outlets. It exceeded my expectations, particularly since my expectations had been lowered by some of the other reviews. It would certainly have been better (for me) had it been longer and with more, and more widely-spaced, outlets, but I can't fault them for making what they made, and their claims seem to have been accurate. Still, the construction is not terribly sturdy and the mounting brackets should have been better thought out.
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Reviewed in the United States on February 2, 2026
★★★★★ 5
Plenty of Outlets and Solid Build
Size: 6ft, Color: Silver
I now have way more outlets than I’ll ever need—haha—but that’s not a bad problem to have! This power strip fits neatly behind my TV stand and feels very well made. The spacing between outlets is convenient for larger plugs, and it seems built to last. Overall, a high-quality and practical solution for managing multiple devices.
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Reviewed in the United States on November 13, 2025
★★★★★ 5
Good for dc power supplies
Size: 6ft, Color: Yellow, Size: 6ft, Color: Yellow
The AC outlets are very snug. I’m using this primarily for battery tenders and battery chargers; the outlets are spaced far enough apart to fit the dc power supplies and they stay in place even when mounted horizontally.
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Reviewed in the United States on December 16, 2025
★★★★★ 5
As good or better than most.
Size: 4ft, Color: Silver
I was very happy with this item. It’s very heavy duty and appears to be something that will last me forever.
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Reviewed in the United States on May 25, 2026