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Ships within 48 hours · Estimated delivery Sep 29 - Oct 4
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Description
PureRec Duplex-Specific Nuclease (DSN)PureRec Duplex Specific Nuclease (DSN) is an endonuclease that selectively digests double stranded DNA (dsDNA) and DNA in DNA RNA hybrids, exhibiting virtually no activity towards single stranded DNA (ssDNA) or RNA. It can discriminate between perfectly matched and non matched short DNA duplexes. To catalyze cleavage, DSN needs a minimum of 10 bp dsDNA or 15 bp perfectly matched DNA RNA duplex. This enzymatic function is reliant upon the presence of
PureRec Duplex-Specific Nuclease (DSN) is an endonuclease that selectively digests double-stranded DNA (dsDNA) and DNA in DNA-RNA hybrids, exhibiting virtually no activity towards single-stranded DNA (ssDNA) or RNA.
It can discriminate between perfectly matched and non-matched short DNA duplexes. To catalyze cleavage, DSN needs a minimum of 10 bp dsDNA or 15 bp perfectly matched DNA-RNA duplex.
This enzymatic function is reliant upon the presence of Mg2+ ions, with a recommended concentration of 5 mM for most applications.
PureRec DSN is overexpressed and extensively purified from a non-animal source and is free of non-specific nucleases or RNases.
Shipping Notes
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Exchange/Return Notes
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