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Description
Mouse & Rat C3 competitive OneStep ELISA KitProduct Specification Antigen C3 Immunogen Recombinant Protein Antibody Type Recombinant mAb Purification Protein A Stability & Storage 12 months from date of receipt reconstitution, 2 to 8'C as supplied. Kit Precision Intra assay: 6. 1% Inter assay: 10. 8% Sample type Cell culture supernatant, Serum, Plasma Assay type Competitive Sensitivity 0. 004 ug mL Range 0. 031 ug mL 2 ug mL Recovery Cell culture supernatant: 113% Serum: 108% Plasma: 102% Assay
Product Specification
| Antigen | C3 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 6.1%； Inter-assay: 10.8% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Competitive |
| Sensitivity | 0.004 ug/mL |
| Range | 0.031 ug/mL – 2 ug/mL |
| Recovery | Cell culture supernatant: 113% Serum: 108% Plasma: 102% |
| Assay time | 60 minutes |
| Species reactivity | Ms;Rt |
Background
Mouse C3 is a central and essential component of the complement system, acting as the pivotal convergence point for all three complement activation pathways: classical, lectin, and alternative. This ~186 kDa protein, primarily synthesized in the liver, is proteolytically cleaved into two key fragments upon activation: the anaphylatoxin C3a, which recruits and activates immune cells, and the opsonin C3b, which tags pathogens for phagocytosis and amplifies the complement cascade. Its critical roles include mediating innate immune defense against infections, clearing immune complexes and apoptotic cells, and bridging innate and adaptive immunity. Consequently, Mouse C3 is a major focus of research in infectious disease, autoimmunity (e.g., lupus), neuroinflammation, and ischemia-reperfusion injury, with genetically modified C3-deficient mice being a fundamental tool for elucidating its precise functions in vivo.
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