SKU: 12507470451

Mouse IL-33 High Sensitivity ELISA Kit

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Description

Mouse IL-33 High Sensitivity ELISA KitProduct Specification Species Reactivity Mouse Synonym C9orf26, C9orf26chromosome 9 open reading frame 26 (NF HEV), DKFZp586H0523, DVS27, DVS27 related protein, IL1F11, IL 1F11, IL33, IL 33, interleukin 33, Interleukin 1 family member 11, interleukin 33, NFHEV, NF HEV, NF HEVNFEHEV, Nuclear factor from high endothelial venules, RP11 575C20. 2 Description Detection principle: This kit uses double antibody sandwich ELISA Technologies. Specificity anti

Product Specification

Species Reactivity Mouse
Synonym C9orf26, C9orf26chromosome 9 open reading frame 26 (NF-HEV), DKFZp586H0523, DVS27, DVS27-related protein, IL1F11, IL-1F11, IL33, IL-33, interleukin 33, Interleukin-1 family member 11, interleukin-33, NFHEV, NF-HEV, NF-HEVNFEHEV, Nuclear factor from high endothelial venules, RP11-575C20.2
Description

Detection principle:

This kit uses double antibody sandwich ELISA Technologies. Specificity anti- Mouse IL-33 capture antibody Pre-coated on high affinity enzyme plates. The standard substance and the sample to be tested are sequentially added to the well of the enzyme label plate , Thoroughly shake and mix, and then put it at room temperature 2 Hours of incubation process, present in the sample IL-33 Binding to solid phase antibodies. After thorough washing to remove free and unbound components, biotinylated detection antibody is added for incubation. After washing again, horseradish peroxidase-labeled streptavidin was added (Streptavidin-HRP , SA-HRP) 。 After washing, the signal enhancer was added for incubation, and after washing to remove unbound material, it was added again SA-HRP 。 After washing again, add TMB The chromogenic substrate is incubated at room temperature in the dark from light to develop color. The depth of color reaction and the sample IL-33 The concentration is positively correlated. Add a stop solution to stop the reaction, and use a microplate reader to 450 nm Detection wavelength ( Correction wavelength 570-630 nm) The absorbance values were determined under conditions.

 

Test Type: Double antibody sandwich method

 

Form: Pre-coating 96 Orifice plate

 

Test Sample Type: Cell supernatant, serum, plasma

 

Sample Load: 100 μl

 

Kit Components: Pre-coating 96 Well plate, standard, biotin labeled IL-33 Test antibody, standard dilution, test buffer, signal enhancer, signal enhancer dilution, TMB A chromogenic substrate, a wash liquid, a stop liquid, SA-HRP , a copy of the sealing film and instructions.

 

Sensitivity: 0.26 pg/mL

 

Detection range: 7.81-500 pg/mL

 

Recovery Range: 80-102%

 

Save Method: 2-8℃

 

Standard graph:

 

 

Background:

Interleukin 33 (IL-33) Also known as NF-HEV And DVS 27 , yes IL-1 Family members, with a molecular weight of 30 kDa , by more than Various types of cell secretion, including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells and Epithelial cells. IL-33 Inducible adjuvant T Cells, mast cells, eosinophils, and basophils secretion II Type cell Factor. IL-33 By interacting with the receptor ST2 And IL-1 Receptor accessory protein (IL1RAP) Binding, activation can promote polarization Th2 Cells secretion II Type cytokine ( Such as IL-5 And IL-13) Of NF-κB And MAP Extracellular molecules in the kinase signaling pathway to mediate their biological Learning function. Injection IL-33 After, it can be induced in vivo II Type cytokines, which are thought to induce severe pathological changes in mucosal organs.

Storage Temp. Stored at 2 ~ 8 ℃, the validity period is two years.
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SKU: 12507470451

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4.2 ★★★★★
Based on 14 reviews
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W. Dietrich
Lexington, US
★★★★★ 5
Great Toy For a Great Price
Color: Purple
My Aussie loves toys and plays with them as though she is a small child. She has a basket and will go to the basket and dig through it until she finds what she is looking for. Lately she has been using the bungee gecko as her toy of choice. She gets bored easily and will rip the stuffing out of most toys, the fact that this one has lasted 6 weeks or so is impressive with no rips, holes or missing limbs. Her favorite way to play is to come over to me and nudge me to play tug of war and we have broke many toys this way but so far we can play tug a war several minutes at a time and the gecko is still in one piece. For the price which is considerable less than some of her other toys I have already ordered a couple of replacements for the future. This is a great well built dog toy that has has a lot of give and can be stretched without ripping.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 3, 2013
Y
Verified Purchase
ynk
Pawtucket, US
★★★★★ 3
No longer as durable
Color: Purple
I have one of these that has made it through 2 dogs but now the bungee is stretched out. It's been such a well loved toy I recently got another one. They've changed the plush fabric. The old one was indestructible. This new one is much thinner and easily torn. I wish they would go back to the old plush fabric.
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Reviewed in the United States on February 4, 2024
D
Verified Purchase
Donna
Natrona Heights, US
★★★★★ 5
Great Toy!
Color: Orange
This has been a great toy for our 11 week old German Shepherd. She absolutely loves it. I can get out all of her toys and lay them out with the exception of her "Lizzy" and she will immediately go to her toy bin and get it herself. Needless to say it is her favorite toy. She has had it a week now and she plays with it a lot, chewing and squeaking. It's a pretty amazing toy to have held up this long already and to date shows no sign of wear or damage. Lizzy just keeps going and going.
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Reviewed in the United States on April 4, 2014
A
Verified Purchase
Anna Rita
Lowell, US
★★★★★ 1
This item came with plush missingand thread unraveling on one leg
Color: Blue
I bought this toy because my dog spent a week playing with the same toy from Zanies that belonged to a visiting dog. My dog was very gentle with this toy, which he loved, so we decided to find one for him. The toy that arrived had no Zanies label and missing plush on the bottom where the label should have been, plus other bare spots in the area that showed the white mesh beneath. It also had a long thread at the joint of a rear leg so I knotted it at the joint and clipped it to prevent my dog from pulling at it but it's apparent that the sewing is loose and wont last long. The color was also much lighter than the toy he'd been playing with that belonged to the guest dog but I figured it might just be from a different dye lot (it was not bought at Amazon but their local pet store.) I am not convinced this is a real Zanies toy. There were two labels in French and it said it was made in China. I am returning this toy and will get a replacement and see how it looks. If it seems like it will fall apart or is not the real thing I will return it immediately. If it has a label and is in good shape and sewn well at the seams I'll revise this review.
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Reviewed in the United States on August 4, 2020
N
Verified Purchase
Neil Ginsberg
Fort Morgan, US
★★★★★ 5
Fantastic Dog Toy!!!
Color: Purple
This is a great toy!!! My dog has lots of toys. But one of the problems is he has trouble getting the squeaker to squeak. He's an 18 lb terrier mix, and those squeakers are just elusive. Breaks my heart. This one, though, the squeakers (there are two) are in small areas (head and tail), that are easy for him to get his whole mouth around and squeak. They also don't move, which also makes them easy to squeak. So he's been squeaking up a storm since he got this! Other plusses: * The material is great (soft, furry material, that seems to be of good quality). * The toy expands and contracts, which is fun. * It's pleasant to look at. All in all, I'd say this is one of the best dog toys I've ever bought for my dog. Highly recommended!
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Reviewed in the United States on January 11, 2015

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