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Description
Human Cervical Cancer Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm<(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)<(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion<(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4  & Nbsp& Nbsp& Nbsp& Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A. |
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| Description | Composition:
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| Storage Temp. | 4° C. 3months-20° C. 1 year, see label for details |
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4.3 ★★★★★
Based on 17 reviews
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Product Reviews
★★★★★ 4
Great for short term
Color: Storage Cover Black, Size: Rechargeable
Works great when it works.... But I've bought three this year and two of them just unexpectedly died with no logical reason. Giving up and trying a new brand for my protein coffee drinks. *Edit....after original review the seller offered a refund and apologized for the issues I had. Bumping up my rating for proactive customer service and trying to make things right.
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Reviewed in the United States on May 23, 2026
★★★★★ 5
Great buy!
Color: Storage Cover Black, Size: Rechargeable
Great product. I'm glad I bought this to replace my old, cheap one that I got from Ikea. I like that it has double whisk as it does froth my liquids really well. I like the speed of it and it's easy to use and wash. The battery life is pretty long as well. I haven't charged it since I got it.
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Reviewed in the United States on June 4, 2026
★★★★★ 5
Pretty darn good frother...
Color: Storage Cover Black, Size: Rechargeable
This is a pretty darn good frother/whisk, which we use primarily for mixing Crystal Light and LMNT drinks. It holds a charge for a long time and it is easy to recharge with the USB-C port and cable. My only complaint is that you must hold the power button continuously while operating it which is challenging to do using tall cups. It would be much better if is was a two stage on-off switch and had a longer wand, which isn't as long as it could be. But the unit is lightweight, easy to use and very versatile. Overall, I would buy it again.
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Reviewed in the United States on April 15, 2026
★★★★★ 5
Works great!
Color: Storage Cover Black, Size: Rechargeable
I bought this because the frother I had was very weak. Even with new batteries, it just never had much oomph. I liked the idea of it being rechargeable and no more batteries!
I expected to have to charge it when it came, but it had full power. I have used it every day since it arrived, and it hasn't slowed down a bit. It is easy to use, just pushing down with my thumb on the top. I also love that it has a lid/case. When I bring it on a trip it is fully protected from damage.
I highly recommend this frother. It is the best one I have had - and I have had quite a few.
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Reviewed in the United States on April 16, 2026
★★★★★ 5
Works way better than its price would suggest
Color: Storage Cover Black, Size: Rechargeable
A family member got frothed milk in their coffee and loved it, so they started doing it at home. They were using a wand mixer which is great at splashing milk all over creation but not very good at actually frothing it.
I asked them what kind of frother they wanted and they gave me 2 criteria: Rechargeable, and inexpensive. They didn't want to spend a lot on something they might not like or might outgrow, and they didn't want to constantly be looking for batteries.
This frother fits the bill. It recharges with a regular USB-C cable and comes with a short one (but not a wall plug so make sure you have one of those). It was also less than $11 delivered so not a lot to gamble on an occasional-use gadget.
Does it work? Absolutely, and a lot better than I ever thought it would. I was given a demonstration and in 10 seconds I saw a small cup of half and half turn into frothy foam. I'll admit, it does taste pretty good in coffee.
I 3D printed a stand to hold the mixer upright on the counter. Otherwise the base is too small and it tips over. That's not a complaint, just an observation. If you want it to stand upright, you'll have to do something similar. Otherwise, this thing is a good combination of simple, inexpensive, and effective.
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Reviewed in the United States on June 1, 2026