SKU: 94069533944

AO/EB Double Staining Kit

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Description

AO/EB Double Staining KitProduct Specification Usage Self prepared material: Fluorescence microscope; PBS; Cell counting board; Glass slide; Cover glass slides 1. Preparation of staining working solution Before the formal experiment, take acridine orange staining solution (reagent A), ethidium bromide staining solution (reagent B), and dilution buffer solution (reagent C), and dilute them to the required AO EB staining working solution in the ratio of A: B: C=1: 1: 8 2. Cell

Product Specification

Usage

Self prepared material:
Fluorescence microscope; PBS; Cell counting board; Glass slide; Cover glass slides

1. Preparation of staining working solution
Before the formal experiment, take acridine orange staining solution (reagent A), ethidium bromide staining solution (reagent B), and dilution buffer solution (reagent C), and dilute them to the required AO/EB staining working solution in the ratio of A: B: C=1:1:8
2. Cell preparation
Adherent cells: Digest according to conventional methods. Centrifuge at 1000 rpm for 5 minutes, collect cell precipitates, and wash once with PBS6pieces/ml
3. Staining
Every 25μ Add 2&mu to the cell suspension; Freshly prepared AO/EB staining working solution, gently mix evenly. Incubate at room temperature for 5-10 minutes
4. Observation
Take a clean slide and add 5-10&mu dropwise; Cover the stained cell suspension with a cover glass. Observe and count using a fluorescent microscope's luciferin filter and a 60x magnification objective. This measurement should be repeated at least three times, with at least 100 total cells observed each time

Description

  Acridine orange (AO) is a heterochromatic nucleic acid binding dye with cell membrane permeability. It binds to DNA or RNA by insertion or electrostatic attraction. When combined with double stranded DNA (dsDNA), it emits green fluorescence (ex/em=502/525nm), and when combined with single stranded DNA (ssDNA) or RNA, it emits red fluorescence (ex/em=460/650nm), with a small amount of binding showing orange red fluorescence. Ethidium bromide (EB) is a polycyclic aromatic hydrocarbon fluorescent small molecule and nucleic acid insertion agent, which is chimeric into the base pairs of double stranded DNA or RNA. It has no base specificity and shows red fluorescence ((ex/em=518/605nm). EB is not cell membrane permeable
  Acridine orange (AO) and ethidium bromide (EB) are often used in combination to observe the changes of nucleus and the formation of apoptotic bodies, which are the characteristics of apoptosis. The combination of the two can distinguish living cells, apoptotic cells and necrotic cells. The working principle is that acridine orange can stain both live and dead cells, while EB can only stain cells that have lost membrane integrity. The living cells are uniformly green. Early apoptotic cells are green, and bright green dots are seen in the nuclear energy of cells due to chromosome condensation and nuclear breakage. Late apoptotic cells will also be stained with EB and thus become orange red, but compared with necrotic cells, late apoptotic cells will have concentrated and often broken nuclei. Necrotic cells are also stained orange red, but will have a nuclear morphology similar to that of living cells, without condensed chromosomes.

Product Components:

Component Name Specification Save
Component A Ao stain solution acridine orange dye solution 200ul 2-8 ℃, protected from light
Component B

EB stain solution ethidium bromide dye solution

200ul 2-8 ℃, protected from light
Component C

Dilution buffer dilution buffer

50ml 2-8 ℃, protected from light
General Notes

1. It is also commonly used to double stain cells with H O echst 33342, which can produce blue fluorescence after binding to dn a through the living cell membrane, and propidiu m iodide, which can only produce red fluorescence after binding to dn a through dead cells
2. If there is a low-temperature centrifuge for centrifugation, the effect is better
3. During operation, pay attention to reducing the exposure time of reagent (a) and reagent (b) to strong light
4. EB solution has certain toxicity, please be careful
5. For your safety and health, please wear lab clothes and disposable gloves

Storage Temp. 2-8 ℃ protected from light, 6months
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SKU: 94069533944

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I ordered these for my son who has neck issues and has gone through so many pillows trying to find one that he liked. They’re heavy, so i was worried that they might be “too” heavy to be able to sleep on comfortably, so I ordered a second set, one firmer than the other (just in case) and gave him a set for Xmas and he loved the first set so much without even trying the less firm ones.He also needs the cooling factor and these offered that, too. They are filled with pieces of memory foam (tons of it) and each pillow can be modified by removing some of the foam. The covers are beautiful, high quality fabric with a zipper so ts easy to remove or replace the foam pieces. The size is perfect and they keep their shape. I kept the second set i ordered rather than returning them and i love the pillows. I actually modified two by removing some of the foam pieces. I kept the foam pieces in a zippered pillow protector and i can always adjust mine when or if i need to. 1000% love these.
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Reviewed in the United States on March 22, 2026
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Asha
Birmingham, US
★★★★★ 5
The Perfect Firm Pillow l
Size: Queen (1 Count), Color: Original White (Cooling)
It is everything it claims to be, cool, comfortable and strong. I got this for my husband first, he loves it and then got one for my son, he uses it to support his knees and has been sleeping much better since then. They both needed a firm pillow that is not super soft, firm but not achy. Finally found the perfect one.
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Reviewed in the United States on May 6, 2026
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Los Angeles, US
★★★★★ 5
This Pillow Is A Must Buy!! What Are You Waiting For?
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Reviewed in the United States on June 4, 2026
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ahindris
San Leandro, US
★★★★★ 5
This is a high-quality, well-designed pillow that combines softness, comfort, and adjustability
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I purchased the DreamyBlue Signature Adjustable Pillow for my husband because he often experiences back discomfort, and many of the pillows we had previously would become flat and lose their support over time. This pillow has exceeded our expectations. It is incredibly soft, comfortable, and comes with extra filling, which allows you to adjust the firmness to your personal preference. We really appreciated having that option. Even after extended use, the pillow has maintained its shape much better than other pillows we've owned. It provides a plush, cozy feel while still offering good support. My husband finds it very comfortable, and it has quickly become his favorite pillow. This is a high-quality, well-designed pillow that combines softness, comfort, and adjustability. I would definitely recommend it.
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Finally Found a Pillow Worth Keeping
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I genuinely feel like I’ve tried every pillow on the market. At one point, I thought Amazon was going to flag my account because I bought and returned so many pillows trying to find the right one. This is the only pillow that made the cut. The shredded memory foam filling makes a huge difference because the pillow is both soft and supportive, while still being adjustable to fit the way you sleep. It arrives extremely full and plush, and it even comes with an extra bag of fill, which honestly surprised me. I ended up removing about half of the filling to get it exactly how I wanted it. I’m a side/stomach sleeper, which can make finding a good pillow difficult since most are either too thick or too flat. This one was perfect because I could shape it exactly how I sleep. It stays cool throughout the night, has a slightly heavier premium feel, and feels much higher quality than most pillows I’ve tried. Super comfortable, supportive, and customizable. I would absolutely recommend it and would definitely buy it again
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