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Description
Cleaved PARP1 Recombinant Rabbit mAb,PBS Only (SDT-R084)Product Specification Host Rabbit Antigen Cleaved PARP1 Synonyms Poly [ADP ribose] polymerase 1, ARTD1, ADPRT 1, msPARP Immunogen N A Location Nucleus, Nucleolus Accession P11103 Clone Number SDT R084 Antibody Type Rabbit mAb Application WB, IHC P, IP, ICFCM Reactivity Hu, Ms Purification Protein A Concentration 1 mg ml Physical Appearance Liquid Storage Buffer PBS Stability & Storage 12 months from date of receipt, 4C as supplied Dilution application
Product Specification
| Host | Rabbit |
| Antigen | Cleaved PARP1 |
| Synonyms | Poly [ADP-ribose] polymerase 1, ARTD1, ADPRT 1, msPARP |
| Immunogen | N/A |
| Location | Nucleus, Nucleolus |
| Accession | P11103 |
| Clone Number | SDT-R084 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P, IP, ICFCM |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS |
| Stability & Storage | 12 months from date of receipt, 4°C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:4000 | |
| IHC-P | 1:1000 | |
| ICFCM | 1:250 | |
| IP | 1:25 |
Background
PARP1 possesses a nuclear localization sequence (NLS) near the DNA-binding domain and a caspase-cleavage site between the DNA-binding domain and the automodification domain [PMID: 10455009]. During caspasedependent apoptosis, PARP1 is cleaved by caspases 3 and 7 at its caspase-cleavage site into 24-kDa and 89-kDa fragments [PMID: 10497198, PMID: 8358726]. The 24-kDa PARP1 fragment contains the DNAbinding motif and the NLS, whereas the 89-kDa PARP1 fragment contains the automodification and catalytic domains. After PARP1 cleavage by caspase, the 24-kDa PARP1 fragment irreversibly binds to DNA breaks and acts as a transdominant inhibitor of active PARP1, whereas the 89-kDa PARP1 fragment is translocated to the cytoplasm [PMID: 11570811, PMID: 9721847]. Thereby, PARP1 fragmentation by caspase leads to its inactivation, which inhibits DNA repair and facilitates caspase-mediated DNA fragmentation in apoptosis.
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