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Description
Human Gastric Cancer Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) the tissue after sampling must be placed in the pre cooled (2 8 c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information (2) prepare several Petri dishes and add 4 pre cooled primary culture buffer B for standby (3) disinfect the sampling bottle, put the tissue into the Petri dish,
Product Specification
| Usage |
1. Primary (1) the tissue after sampling must be placed in the pre cooled (2-8° c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information (2) prepare several Petri dishes and add 4 ℃ pre cooled primary culture buffer B for standby (3) disinfect the sampling bottle, put the tissue into the Petri dish, wash it with primary culture buffer B for three times, and then cut the tissue into a tissue block with a volume of about 1-3mm3using ophthalmic scissors or scalpel (4) the tissue was digested with human gastric cancer primary tissue digestive solution C, and then it was shaken at 37 ℃ for 10-20min (the digestion condition was observed at any time during the digestion process) (5) take a small amount of liquid and observe it under the microscope. After observing more single cells or cell clusters below 70UM under the microscope, add three times the volume of primary culture buffer B to stop digestion (6) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation (7) Matrigel calculation: after step 6, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate (8) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃) (9) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a gel, and add human gastric cancer organoid medium a (restore room temperature) for culture 2. Organoid subculture (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4  &Nbsp&Nbsp&Nbsp b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4 (4) after organoids were collected, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul human gastric cancer organoid culture medium a 3. Organoid cryopreservation (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid (4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube (5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage 4. Organoid resuscitation (1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube (2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting (3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min (4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g (5) resuspend the Matrigel, lay 25ul of Matrigel per well in a 24 well cell culture plate, place it in an incubator for 10-15min to form a gel, and add 500ul of human gastric cancer organoid culture medium A. |
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| Description | Composition:
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| Storage Temp. | 4° C. 3months; -20° C. 1 year, see label for details |
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4.5 ★★★★★
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Product Reviews
★★★★★ 5
Perfect cutting board set of 3
Very nice cutting boards, and I especially love the peace of mind knowing there is not harsh chemicals used to make these. Thank you!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 27, 2026
★★★★★ 5
Fascinating exploration of God's Word!
Urban Legends of the New Testament: 40 Common Misperceptions is an impressive work by David A. Croteau that discusses 40 "commonly circulated myths, repeated throughout the culture as common knowledge, but which isn't true."
But we're talking about the infallible Word of God, right? That is correct. But according to the author, certain passages of the New Testament have become victims of the notorious Urban Legend phenomena.
The titles of each chapter are the actual legends and are presented as if the author really believes what he is writing to be true. The reader will soon find out that is not the case. Croteau goes on to discuss the incorrect interpretation versus an accurate interpretation and then ends the chapter with his opinion of what the text means, followed by a practical application section.
The presentation and discussion of each myth is clear, although some are harder to piece through than others. The length of the chapters vary according to the history of the myth and depth of the discussion. Topics vary from the birth, life, and crucifixion of Jesus to Paul's profession, the plan of salvation, hell, divorce, money, pastors and their families, and cults. You can pick up the book and read only the chapters that interest you, although I do encourage you to read the introduction and epilogue.
The prologue discusses two types of legends: a mistaken-contains wrong information; misleading-those that need clarification. The cause and solution for urban legends of the New Testament come down to three components: Context, Greek, and Backgrounds. Croteau clearly discusses this at the end of the book and my only criticism is that I would have liked that information in the introduction. I believe it would have aided in my overall structure for comprehension.
Overall, the research was fascinating and I learned—a lot.
This quote from the final conclusion summarizes the purpose of this book:
"In the end our goal should be to interpret Scripture as accurately as possible, to apply that interpretation to our own lives, and to be transformed by God's Word so that our lives may bring glory to the One who rescued us from an eternal destiny in hell."
I highly recommend this book to all readers. Don't plan to read it in one sitting. Use it as a study guide and tool to inspire you to dig deeper into God's Word.
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Reviewed in the United States on January 12, 2016
★★★★★ 5
Only Orthodoxy Will Lead to Orthopraxy and Orthopathy
Format: Kindle
This book authored by David Croteau is truly an enjoyable yet at the same time a convicting read. Even though it was written about 10 years ago, the principles of interpretation advocated by him to deal with the "mistaken and misleading legends" are timeless. Out of the 40 legends, I have encountered at least half of them in the local church, writings and even seminary especially issues pertaining to salvation, Christian living and women's adornment.
The main takeaway is this: Just as there are three things that matter in property: "Location, Location, Location", similarly the three main things that matter in interpretation of the Word is: "Context, Greek, and Background Information", or even simpler: "Context, Context, Context". David Croteau has demonstrated in his 40 legends the utmost importance of context in correctly interpreting a particular passage which was unfortunately taken out of context. The interpretation can further be handled even more accurately by taking into account the Greek language and the socio-historical background behind the text. In fact, I have been focusing on these three elements in my hermeneutical approach whenever I come across passages which I need more understanding. I don't know Greek; but with the many useful online Greek tools nowadays, I can always do a word study which more or less balances my lacking of Greek knowledge. Also, the study of socio-historical background is extremely illuminating if one have access to these resources. Personally, I discover that a proper understanding of background information can eliminate most of the mistaken or misleading interpretation of a text.
Lastly, I totally agree with David Croteau's conclusion: "In the end our goal should be to interpret Scripture as accurately as possible, to apply that interpretation to our own lives, and to be transformed by God’s Word so that our lives may bring glory to the One who rescued us from an eternal destiny in hell." His conclusion reminds me of a phrase which I encountered years ago: "Orthodoxy Will Lead to Orthopraxy and Orthopathy." Indeed, only by "accurately handling the word of truth" (2 Tim 2:15) can we realise the necessity of holy living and the proper response that we should have towards our living God.
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Reviewed in the United States on April 6, 2025
★★★★★ 5
The book is also great in pointing to additional resources for further study
Format: Paperback
Other than the Bible, I hesitate to use the term "must read" for a book. This one is definitely a "should read" for anyone who teaches, preaches, or wants to minister more faithfully. The book is engaging, and offers both a negative (doesn't mean this) and positive (means this) response to common urban legends of the NT. You'll be surprised by some beliefs you had that you didn't even realize were not correct, or should be adjusted. The book is also great in pointing to additional resources for further study. Pastors, seminarians, and lay people will all benefit from this book.
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Reviewed in the United States on July 17, 2015
★★★★★ 5
Rescuing the Bible from its Friends
The inerrant, infallible Word of God has endured countless intentional assaults of misrepresentation by its enemies. Unfortunately it has also had to endure the unintentional insults of misinterpretation by its friends. Dr. Croteau has given us an excellent handbook to gently correct. His specific examples are well considered and his general principles are well established. Highly recommended.
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Reviewed in the United States on August 19, 2015
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