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Description
Human Thyroid Stimulating Hormone OneStep ELISA KitProduct Specification Antigen Thyroid Stimulating Hormone Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Hu Purification Protein A Stability & Storage 12 months from date of receipt reconstitution, 2 to 8'C as supplied. Kit Precision Intra assay: 3. 6% Inter assay: 4. 4% Sample type Cell culture supernatant, Serum, Plasma Assay type Sandwich (quantitative) Sensitivity 2. 135 pg ml Range 7. 81 pg mL 500 pg mL Recovery Cell
Product Specification
| Antigen | Thyroid Stimulating Hormone |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 3.6%； Inter-assay:4.4% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 2.135 pg/ml |
| Range | 7.81 pg/mL – 500 pg/mL |
| Recovery | Cell culture supernatant: 111% Serum: 100% Plasma: 102% |
| Assay time | 60 minutes |
| Species reactivity | Human |
Background
Human Thyroid Stimulating Hormone (TSH), a glycoprotein hormone secreted by the anterior pituitary gland, plays a pivotal role in regulating thyroid function by stimulating the synthesis and secretion of thyroid hormones (T3 and T4), which are essential for metabolism, growth, and development. TSH binds to TSH receptors on thyroid follicular cells and its secretion is regulated by a negative feedback loop involving thyrotropin-releasing hormone (TRH) from the hypothalamus and circulating T3/T4 levels. As the most sensitive marker for thyroid disorders, elevated TSH indicates hypothyroidism while suppressed TSH suggests hyperthyroidism. With a molecular weight of ~28 kDa consisting of α- and β-subunits, TSH is typically measured using immunoassays (ELISA, CLIA, RIA) with a normal range of 0.4-4.0 mIU/L. Clinically, TSH testing is indispensable for diagnosing various thyroid conditions (Hashimoto's thyroiditis, Graves' disease), monitoring levothyroxine therapy, and assessing thyroid function during pregnancy, making it the cornerstone of thyroid function evaluation due to its high diagnostic reliability.
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