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Description
Cys-Protein GProduct Specification Amino Acid Sequence Leu298 Glu495 with an N terminal Cys Expression System E. coli Molecular Weight Approximately 21. 9 kDa, and it migrates with an apparent molecular mass of 40 kDa in SDS PAGE. Purity 95% by SDS PAGE or HPLC. Conjugation Unconjugated Physical Appearance Lyophilized powder Storage Buffer PB, pH 7. 4. Reconstitution Before use this product, please read the direction below carefully. This vial must be briefly
Product Specification
| Amino Acid Sequence | Leu298-Glu495 with an N-terminal Cys |
| Expression System | E.coli |
| Molecular Weight | Approximately 21.9 kDa, and it migrates with an apparent molecular mass of 40 kDa in SDS-PAGE. |
| Purity | ＞95% by SDS-PAGE or HPLC. |
| Conjugation | Unconjugated |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PB, pH 7.4. |
| Reconstitution | Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage | For long term storage, the product should be stored ≤ -20℃. |
Background
Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcic. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F (ab') fragments of IgG. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology Recombinant Cys-Protein G is a single non-glycosylated polypeptide chain containing 201 amino acids. and there is an N-terminal cysteine for Site-Specific Conjugation.
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