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Description
Alexa Fluor® 647 Rat Anti-Mouse Ki67 Antibody (S-R595)Product Specification Host Rat Antigen Ki67 Synonyms Proliferation marker protein Ki 67; Antigen identified by monoclonal antibody Ki 67 homolog (Antigen KI 67 homolog; Antigen Ki67 homolog); Mki67 Location Nucleus Accession E9PVX6 Clone Number S R595 Antibody Type Rat mAb Isotype IgG2a,k Application ICFCM Reactivity Ms Positive Sample NIH 3T3 Purification Protein G Concentration 0. 05mg ml Conjugation Alexa Fluor 647 Physical Appearance Liquid
Product Specification
| Host | Rat |
| Antigen | Ki67 |
| Synonyms | Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog (Antigen KI-67 homolog; Antigen Ki67 homolog); Mki67 |
| Location | Nucleus |
| Accession | E9PVX6 |
| Clone Number | S-R595 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | ICFCM |
| Reactivity | Ms |
| Positive Sample | NIH/3T3 |
| Purification | Protein G |
| Concentration | 0.05mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICFCM | 5μl per million cells in 100μl volume | Ms |
Background
Ki67 protein is a non-histone nuclear protein encoded by the MKI67 gene, widely used as a proliferation marker in pathology. It is expressed during all active phases of the cell cycle—G1, S, G2, and M—but is absent in quiescent (G0) cells. Ki67 has multiple molecular functions, including forming the perichromosomal layer to prevent chromosome aggregation during mitosis and organizing heterochromatin. It also facilitates chromosome attachment to the mitotic spindle and individual chromosome mobility. In addition to its role in cell division, Ki67 is a valuable prognostic and predictive indicator in cancer diagnosis, with its expression levels correlating with tumor cell proliferation rates.
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