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Description
Mouse Bax ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000 × g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with ice-cold PBS and resuspend them in 150-200 μL of PBS per 1 × 10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500 × g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Bcl2 Associated X Protein (Bax) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Bcl2 Associated X Protein (Bax) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Bcl2 Associated X Protein ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Bcl-2-associated X protein (BAX) is a protein encoded by the BAX gene. BAX is a member of the BCL-2 gene family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators, participating in various cellular processes. This protein forms a heterodimer with BCL2 and functions as an apoptotic activator. BAX is the first identified pro-apoptotic member of the Bcl-2 protein family. Bcl-2 family members share one or more of four characteristic homology domains, designated the Bcl-2 homology (BH) domain (BH1, BH2, BH3, and BH4), and can form hetero- or homodimers. These domains are composed of nine α-helices: a hydrophobic α-helical core surrounded by amphipathic helices and a transmembrane C-terminal α-helix anchoring the protein to the mitochondrial outer membrane. A hydrophobic groove formed along the C-terminus of α2 to the N-terminus of α5, along with some residues in α8, binds to the BH3 domains of other BAX or BCL-2 proteins in their active form. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates, cell lysates and other biological fluids |
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4.4 ★★★★★
Based on 18 reviews
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Product Reviews
★★★★★ 5
Premium wrist rest for a premium price
Color: Right - Black, Size: Small
Does as described. I replaced my old stationary gel wrist pad with this. There is an adjustment period to get used to laying your wrist back into it after typing but I can definitely say my wrist isn't as sore nor has that tingly carpal tunnel sensation came back after a heavy gaming session. Glides pretty smoothly on my mousepad but I'm not sure how it is on cloth pads. Worth the investment if you have the money to spend on it. Also the packaging definitely feels luxurious.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 15, 2026
★★★★★ 4
Beautiful design, noticeably less wrist pain
Color: Right - Black, Size: Large, Color: Right - Black, Size: Large
-Packaging:
Simply amazing. The packaging material was great and the box has a nice sliding mechanism. Thoughtful.
The box comes with instructions, a bunch of QR codes on the box for more details, and a nice carrying case.
-The item:
Very low profile and gorgeous design. The item is made of durable material. The 'cushions' on the top side are very comfy, especially around the areas of the wrist that will carry most of your hand's weight. The bottom is designed so that your hand can glide along with your mouse with minimal friction (using with fabric mouse pad).
-Uses:
1. Work: it is a must-have if you have an office job. I have been using mine for a few days at work and I feel much less pain in my wrist.
2. Gaming: It does get a while to get used to it, but after a while the movement becomes natural and you won't notice the Carpio
Just like with using it for work, you will not feel wrist pain after long gaming sessions.
I believe my aim in fps games got just a bit better while using the item. Or hey, sometimes my opponent is just not having a really good day.
Cons:
1. A bit overpriced. It may have taken a lot of research and design to come up with the product, but I would have been a lot happier if I had paid just a little less for the product. However, it does beat the competition in both, design and comfort.
2. Using the Carpio is pleasant most of the time, as long as your hand does not leave it, but as soon as you lift your hand off the Carpio it takes too long to find the sweet spot again. The box says it would take two weeks to get used to using the product and I'm only in my first week. I think this will change the more I get used to using the product.
3. For con reasons 1 & 2 above, I think it would not be a good idea to have two Carpios (right-handed and left-handed) to use with the keyboard
Overall, I do recommend this product and love using it so far for work and gaming, but I rated it 4/5 only because of how I feel about the price.
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Reviewed in the United States on February 4, 2023
★★★★★ 5
Excellent Product
Color: Right - Black, Size: Large
Really comfortable. Very usefull for my wrist, great shape, really smooth on the movement. Recommended to take care of you wrist.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 18, 2026
★★★★★ 5
Elevates your wrist
Color: Right - Black, Size: Large, Color: Right - Black, Size: Large
This is a great tool. No wrist pain day 1. It elevates your wrist to level with the mouse so there’s no backward strain. Takes a little getting use to but well worth no pain.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 5, 2026
★★★★★ 3
It's hard af.
Color: Right - Grey, Size: Small, Color: Right - Grey, Size: Small
Edit: just realized Amazon sent me a left handed one!
I got this one and the g2 or whatever with the strap. The g2 is great. This one is hard though. The edges of the rest thingy. And the edges in the middle where it splits are annoying. Idk if it's a difference in the rubber pads or what. Even if this works for wrist pain, it's not worth it for the palm discomfort.
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Reviewed in the United States on April 14, 2026