SKU: 23148770411

Human NKX2.2, His tag

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Description

Human NKX2.2, His tagProduct Specification Species Human Synonyms Homeobox protein NK 2 homolog B, NKX2. 2, NKX2B Accession O95096 Amino Acid Sequence Protein sequence (O95096, Met1 Lys127, with C 10*His) MSLTNTKTGFSVKDILDLPDTNDEEGSVAEGPEEENEGPEPAKRAGPLGQGALDAVQSLPLKNPFYDSSDNPYTRWLASTEGLQYSLHGLAAGAPPQDSSSKSPEPSADESPDNDKETPGGGGDAGKGGGGSHHHHHHHHHH Expression System HEK293 Molecular Weight Predicted MW: 14. 9 kDa Observed MW: 20 33 kDa Purity 95% by SDS PAGE Tag with C

Product Specification


Species Human
Synonyms Homeobox protein NK-2 homolog B, NKX2.2, NKX2B
Accession O95096
Amino Acid Sequence

Protein sequence (O95096, Met1-Lys127, with C-10*His) MSLTNTKTGFSVKDILDLPDTNDEEGSVAEGPEEENEGPEPAKRAGPLGQGALDAVQSLPLKNPFYDSSDNPYTRWLASTEGLQYSLHGLAAGAPPQDSSSKSPEPSADESPDNDKETPGGGGDAGKGGGGSHHHHHHHHHH

Expression System HEK293
Molecular Weight Predicted MW: 14.9 kDa Observed MW: 20-33 kDa
Purity >95% by SDS-PAGE
Tag with C-10*His
Physical Appearance Lyophilized Powder
Storage Buffer Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4.
Reconstitution Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
Stability & Storage

12 months from date of receipt, -20 to -70 °C as supplied. 6 months, -20 to -70 °C under sterile conditions after reconstitution. 1 week, 2 to 8 °C under sterile conditions after reconstitution. Please avoid repeated freeze-thaw cycles.

Background

Homeobox protein Nkx-2.2 is a protein that in humans is encoded by the NKX2-2 gene. Homeobox protein Nkx-2.2 contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor. The expression of Nkx2-2 is regulated by an antisense RNA called Nkx2-2as. In the developing spinal cord, Nkx-2.2 regulates IRX3 thereby contributing to the proper differentiation of the ventral horn neurons.

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SKU: 23148770411

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4.5 ★★★★★
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Verified Purchase
Mike Steven
Charlottesville, US
★★★★★ 5
Quality and Function
Looks like a silly little thing, I am not a coffee enthusiast. But it does exactly what it is supposed to do and with high quality. The device (a stretch to call it that) is made of sturdy materials including the needles. They give you two different thicknesses of needles, one for expressi (0.3) and one set for coffee grinds (0.4). Each comes wth some spares. The stand is well designed and keeps grinds from spreading all over. It is very elegant design. I use this with a dosing funnel on my portafilter. Not the cheapest one could buy but well worth the money.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 16, 2025
S
Verified Purchase
Sunny
West Palm Beach, US
★★★★★ 5
Well made, high quality product.
This product demonstrates excellent quality, featuring a robust construction and intuitive design for ease of use. It includes two distinct wire sizes and offers straightforward assembly. Its superior durability and strong magnetic properties ensure effortless reattachment to its stand.
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Reviewed in the United States on June 30, 2025
A
Verified Purchase
Amazon Customer
Phoenix, US
★★★★★ 5
Perfect WDT tool
Best WDT I’ve found. Love that it includes 0.3mm needles (as I’ve found that 0.4mm needles push the grounds around too much), and the stand is very clever. It knocks off the residual grounds on the needles and is easy to use. As far as WDT tools go, I can’t imagine anything better.
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Reviewed in the United States on April 13, 2025
M
Verified Purchase
MrEdmonds
New York, US
★★★★★ 5
Amazing quality build
This WDT tool is solid and well-made. Definitely worth having!
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Reviewed in the United States on June 26, 2025
A
Verified Purchase
Amazon Customer
Fort Morgan, US
★★★★★ 5
Get The Clumps Out
Nice companion in the Espresso Yard. Watch for the guards, and shiv away at that fresh ground Espresso meat.
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Reviewed in the United States on January 8, 2026

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