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Description
UniOne® TR-FRET Human Epigeneous Bromodomain Binding KitProduct Specification Host Human Stability & Storage 80 Background This kit utilizes Homogeneous Time Resolved Fluorescence(TR FRET) technology to measure the interaction between BRD4 and the[LYS(5,8,12,16)AC] H4(1 21) peptide. This method enables simple and rapidhigh throughput screening of inhibitors and blocking antibodies. As illustrated, the interaction between BRD4 and the[LYS(5,8,12,16)AC] H4(1 21) peptide is detected using a Eu labeled Anti
Product Specification
| Host | Human |
| Stability & Storage | -80℃ |
Background
This kit utilizes Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the interaction between BRD4 and the[LYS(5,8,12,16)AC] H4(1-21) peptide. This method enables simple and rapidhigh-throughput screening of inhibitors and blocking antibodies.
As illustrated, the interaction between BRD4 and the[LYS(5,8,12,16)AC] H4(1-21) peptide is detected using a Eu-labeled Anti-Tag1antibody (TR-FRET donor) and an Acceptor-labeled Anti-Tag2 antibody (TR-FRETacceptor). The binding of BRD4 to the [LYS(5,8,12,16)AC] H4(1-21) peptidebrings the donor and acceptor antibodies into close proximity. Excitation ofthe donor antibody then triggers Fluorescence Resonance Energy Transfer (FRET)to the acceptor antibody, resulting in a specific emission signal at 665 nm.The intensity of this specific signal is proportional to the degree ofinteraction between BRD4 and the [LYS(5,8,12,16)AC] H4(1-21) peptide. Thishomogeneous assay is simple to perform and requires no washing steps.
Components
Table 1. Kit Components and Storage Conditions
Component |
Concentration |
100 TESTS |
500 TESTS |
2500 TESTS |
10000 TESTS |
Storage Temp. |
|---|---|---|---|---|---|---|
(+)-JQ-1 |
300μM |
3μL |
10μL |
50μL |
200μL |
-80℃ |
Tag1-BRD4 protein |
100× |
4μL |
20μL |
100μL |
400μL |
-80℃ |
Tag2- peptide |
100× |
4μL |
20μL |
100μL |
400μL |
-80℃ |
Anti-Tag1 Eu dye antibody |
50× |
10μL |
50μL |
250μL |
1000μL |
-80℃ |
Anti-Tag2 Ac antibody |
12.5× |
40μL |
200μL |
1000μL |
4000μL |
-80℃ |
Detection buffer |
10× |
400μL |
2mL |
10mL |
40mL |
-80℃ |
* Avoid repeated freeze-thaw cycles of reagents. Aliquot upon first thaw and store according to the specified conditions. Shelf life is one year.
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Exchange/Return Notes
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