Mouse GM-CSF High Sensitivity ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti mouse GM-CSF capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The GM-CSF in the sample binds to the solid-phase antibody. After thorough washing to remove free and unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of GM-CSF in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, biotin labeled GM-CSF detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.23 pg/ml

Detection Range: 2.5-160 pg/ml

Recovery Range: 91-112%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Granulocyte macrophage colony stimulating factor (GM-CSF) is a cytokine that regulates the growth of leukocytes. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils)And monocytes. In addition, it is also active against hematopoietic and non hematopoietic lineage cells. GM-CSF is used as a drug to stimulate the production of leukocytes, thereby preventing neutropenia caused by chemotherapy. Recently, people are conducting clinical trials to evaluate the efficacy of GM-CSF as a potential vaccine adjuvant for HIV infected patients. Relevant studies have reported that some patients with cancer (including lung cancer and acute myeloid leukemia) have higher levels of GM-CSF in the serum, and also have higher levels of GM-CSF in the peripheral blood and scale extracts of patients with bronchial asthma.