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Description
Mouse PTGS2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 30 ng/mL). Then dilute to the following concentrations: 30 ng/mL, 15 ng/mL, 7.5 ng/mL, 3.75 ng/mL, 1.875 ng/mL, 0.9375 ng/mL, 0.46875 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 30ng/mL standard working solution into the first EP tube and mix thoroughly to make a 15ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a prostaglandin endoperoxide synthase 2 (PTGS2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of prostaglandin endoperoxide synthase 2 (PTGS2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Prostaglandin Endoperoxide Synthase 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Prostaglandin Glycogenase Synthase 2 (PTGS2), also known as cyclooxygenase-2 or COX-2, is an enzyme encoded by the PTGS2 gene. It converts arachidonic acid (AA) into prostaglandins (PGs). It is a sequential homodimer. Each monomer of the enzyme has a peroxidase and a PTGS (COX) active site. PTGS (COX) catalyzes the conversion of arachidonic acid to prostaglandins in two steps. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.46-30ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.1 ★★★★★
Based on 13 reviews
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Product Reviews
★★★★★ 5
45 minutes assembly -- piece of cake. Nice chair.
Color: Black, Style: With Headrest, Color: Black, Style: With Headrest
Before I finally landed on this chair I read about a million articles with buying advice for ergonomic desk chairs, looked for second hand expensive chairs, read so many Amazon reviews. Obviously you can spend a whole lot of money on an ergonomic desk chair (even used ones), but I really didn't want to.
I just bought this today, so the jury is out on long term wear, ,but I can say that the components feel solid, assembly was easy, it is *very* comfortable so far, and -- even more to the point -- VERY adjustable in so many different ways that physical therapists etc recommended in the articles I read. I am 5 foot 5; in order to put the chair at the right ergonomic height for my particular desk and keyboard, I need a footrest so my feet aren't dangling. But that would be the case with any chair in my setup. The headrest is great, the arms are soft and comfortable, the back support piece is perfect, and everything can be adjusted as you sit in it. Rollers are quiet and stable on laminate floor.
The box was a good size and heavy enough (~32 lbs) to be awkward for an unfit middle aged lady to wrangle down apartment steps; however, the Amazon driver brought it straight to my door. The chair was carefully packed. Whoever wrote the assembly instructions (which are in English and Spanish) for this chair is some kind of technical writing genius -- I have never seen clearer or more easy-to-follow instructions. From slicing open the tape on the box to finished chair was 45 minutes for me. All you need for assembly is included -- also fabric gloves (for protecting your manicure? I guess?) and extra bolts and washers.
Super buy. If I'd known I could get such an affordable and massive upgrade from my previous garbage chair I would have done it long ago. Great value for the money. I rarely write reviews but all the reviews I read helped me make a great decision, so here ya go.
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Reviewed in the United States on October 29, 2024
★★★★★ 5
Comfort and ease
Color: Black, Style: With Headrest
Good chair all the functions work well I don’t really care for the armrest rotating but the fact they lift up an extend is nice. It’s comfortable. The lumbar is good. I don’t spend hours in a chair. It’s nothing fancy but definitely good for the price. Simple to put together seems durable enough.
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Reviewed in the United States on June 1, 2026
★★★★★ 4
Good chair but....
Color: White, Style: With Headrest
I am 20 years old and a college student, I have horrible posture, and I game a lot, so my chair is VERY important to me. This chair was decently okay to assemble, and the initial sitting experience is good. You can feel yourself sink into the seat but not too much which is really nice. The arm rests are tiltable which is AMAZING because I always have a weird stance when I sit so this is perfect for me. However, there is one thing about this chair that is CRITICAL to know before buying. Perhaps this is my own fault, but the back isn't adjustable. What I mean by that is that you can't lean the back of the chair forward, kind of like how you can in a car seat. There is a pillow that you can push further into the back rest with a nob on the back but that barely helps. The headrest is also abysmal because I have to lean so far back into the chair just for my head to hit the head rest. I want a headrest that is always against my head. Not something that I have to tilt my head back into just for my head to hit it. That makes the chair extremely uncomfortable if I am trying to use the headrest. The chair also feels wobbly, I have had previous chairs where over time the gas lift wears out over time and when I sit on the chair the chair falls to the ground because the gas lift doesn't work anymore. However, that takes years of wear and tear. Sitting in this chair makes me feel like it is going to happen way sooner. Also, the picture makes it look way whiter than it is in real life. It is kind of like a white that is getting to a pee color and it is kind of gross looking a little bit but it isn't that bad since it isn't fully that color. Another thing is that crumbs actually get stuck in the tiny holes in the seat cushion so you can't swipe it off your chair they are literally inside of it. I will leave a better review once I had used it for a while. If you are just looking for a nice chair and don't really care to much about the specifics it isn't a bad choice.
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Reviewed in the United States on July 1, 2025
★★★★★ 5
Great for the price
Color: Black, Style: With Headrest, Color: Black, Style: With Headrest
Purchased this chair for my son who does a lot of gaming. He loves it. Took about 30 mins to assemble. Instructions are very easy to follow.
Pros
- very easy to put together
- very comfy back rest with arch adjustment
- can recline
- can adjust height
- very stable
- material is very sturdy
- wheels moved smoothly over carpet
- material is strong
- solid black color
Cons
- limited adjustments with recline
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Reviewed in the United States on May 25, 2026
★★★★★ 5
Good quality and good price
Color: White, Style: With Headrest, Color: White, Style: With Headrest
Very easy to assemble, comfortable, sleek design, and the colours are just as pictured. I can't speak on durability as I just got it. The back support works well. Something I loved is that the head and arms support adjust to your needs.
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Reviewed in the United States on May 26, 2026