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Description
Mouse SYN1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Gently wash adherent cells with pre-chilled PBS, then trypsinize and collect the cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with pre-chilled PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Collect the supernatant for analysis, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a synapsin I (SYN1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of synapsin I (SYN1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Synapsin I ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Synapsin I (SYN1) is the collective name for synapsin Ia and synapsin Ib, two nearly identical phosphoproteins encoded by the SYN1 gene. Synapsin Ia and Ib are similar in length and composition; however, the amino acid sequence of synapsin Ib ends at the C-terminal end of the amino acid sequence of synapsin Ia. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids |
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4.5 ★★★★★
Based on 8 reviews
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Product Reviews
★★★★★ 5
Total GAME CHANGER - incredibly effective toothbrush for dogs
Color: Clear (2-pack)
This silicon finger brush is incredibly easy to use - and so EFFECTIVE (more on that in the last paragraph this review). At first, it seemed a little hard to get on my finger but I simply got my finger wet first under the faucet and it slipped right on with virtually no effort. And once on, it's easy to take off but doesn't slip off accidentally when in use - so - perfect! Quick & simply to clean - rinses off in the sink in seconds, and ready to use the next time. And, my dog not only lets me clean her teeth - she actually like it (this from a dog who used to hate it when I would try to brush her teeth with a regular doggy toothbrush).
This silicon "brush" is incredibly maneuverable - way more so than standard dog toothbrushes or regular finger brushes. So much so it makes it super easy to not only get those normally impossible to reach back molars - AND - even the back sides of the teeth! With a normal dog tooth brush (or finger brush) my dog's clamping down made that impossible. Now, it's not only possible - it's easy!
Our dog's canine teeth had build-up but it was not until I pulled her gums back and looked at her back molars that I saw how bad things had gotten - and we have a young dog! Her back molars not only had build-up but some appeared to be stained a tea colored brown that did not come off with my fingernail. It was pretty disheartening and I felt terrible. How could this happen so quickly? The vet said it was plaque (and not yet tartar) and that it wasn't bad enough to warrant a professional cleaning - yet - but that I needed to do a better job brushing. He said: "I can see exactly where your brushing stops" (referring to the condition on the back molars which I had not been able to do with a regular brush as my dog just wasn't having it).
Tips: here's what's working for us. Instead of squeezing out a pea sized blog of tooth paste onto this brush, I put a tiny dab - that it's - on the very tip of the brush and focus my efforts on two of our dog's worst teeth on one side - on top and then on the bottom. Then I put another small dab and repeat on the other side of her mouth with 4 teeth on that side. Then, I use another small dab and focus work on another group of teeth. All totaled, I'm using maybe 4 to 6 very small dabs of dog toothpaste, probably half the size of pea collectively, perhaps less. That's it. However much you choose to use is up to you - be sure and use an amount that's safe and effective for your dog.
Effectiveness: this works and it works well! In just 3 days using the same vanilla flavored enzymatic toothpaste by CET I was seeing improvement. By day 7, that improvement was so obvious I was actually surprised by how much better they looked. It's now been a little over two weeks and they are looking considerably better! Conservatively, I'm thinking a 50% improvement. Not bad for just 2 weeks, right? I'm actually think I'm going to be able to completely reverse things - we'll see. I'll try and update this review again in a few weeks and let you know how it's looking but in the meantime, I am absolutely thrilled with this brush. I paid $16 and it came 2 in a pack) and the first (and only one) I've been using still looks brand new after it's rinsed off. All in all - this brush is a game changer! I can now brush our dogs back teeth and even the back sides of her teeth, all in less than a couple minutes a day.
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Reviewed in the United States on August 23, 2020
★★★★★ 3
Plastic not a brush
Color: Clear (4-pack)
Love the idea, dont care for the texture. It would be nice if they were soft brush and not plastic.
Would retuen but i missed the window.
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Reviewed in the United States on May 22, 2026
★★★★★ 5
Better than a toothbrush!
Color: Blue (2-pack)
Perfect for brushing my doggies teeth!
Easy to use and my dogs like it better than a toothbrush.
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Reviewed in the United States on June 2, 2026
★★★★★ 5
Great dog toothbrush!
Color: Blue (2-pack)
I’ve been using this product for a year now, and it’s been working great. When paired with vanilla mint enzymatic toothpaste, it effectively cleans my Pomsky’s teeth, including the back teeth. As a result, my fur baby’s teeth remain as white as ever!
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Reviewed in the United States on May 27, 2026
★★★★★ 5
Hair growth
I see results with my hair growth I'm very pleased. There is a noticeable smell to it but dissipates over a short time.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 9, 2026