SKU: 35080491790

Alexa Fluor® 700 Mouse Anti-Human CD33 Antibody (S-R472)

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Description

Alexa Fluor® 700 Mouse Anti-Human CD33 Antibody (S-R472)Product Specification Host Mouse Antigen CD33 Synonyms Myeloid cell surface antigen CD33; Sialic acid binding Ig like lectin 3 (Siglec 3); gp67; SIGLEC3 Location Cell membrane Accession P20138 Clone Number S R472 Antibody Type Mouse mAb Isotype IgG2a,k Application FCM Reactivity Hu Positive Sample Human PBMC Purification Protein A Concentration 0. 2 mg ml Conjugation Alexa Fluor 700 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin

Product Specification


Host Mouse
Antigen CD33
Synonyms Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3 (Siglec-3); gp67; SIGLEC3
Location Cell membrane
Accession P20138
Clone Number S-R472
Antibody Type Mouse mAb
Isotype IgG2a,k
Application FCM
Reactivity Hu
Positive Sample Human PBMC
Purification Protein A
Concentration 0.2 mg/ml
Conjugation Alexa Fluor® 700
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300

Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 5μl per million cells in 100μl volume Hu

Background

CD33, also known as Siglec-3, is a 67 kDa type-I transmembrane sialic-acid-binding immunoglobulin-like lectin composed of an N-terminal V-set Ig domain that mediates glycan recognition, a membrane-proximal C2-set Ig domain, and a cytoplasmic tail harboring two immunoreceptor tyrosine-based inhibitory motifs (ITIMs); upon ligation by α2,3- or α2,6-linked sialylated glycoproteins or the complement component C1q, Src-family kinases phosphorylate these ITIMs, recruiting the SH2-domain-containing phosphatases SHP-1 and SHP-2 that dephosphorylate downstream signaling molecules, thereby suppressing calcium flux, PI3K activity, and activation of myeloid cells including monocytes, macrophages, dendritic cells, microglia, and myeloid-derived suppressor cells, while also modulating NK-cell cytotoxicity and cytokine production; CD33 is highly expressed on committed myeloid progenitors and over-expressed on >90 % of acute myeloid leukemias, making it the target of the antibody–drug conjugate gemtuzumab ozogamicin, and alternative splicing generates a shorter isoform lacking the ligand-binding V domain that can still dampen immune responses; genetically, the rs3865444(C) allele that reduces CD33 surface expression is protective against late-onset Alzheimer’s disease because lower microglial CD33 lessens inhibition of Aβ phagocytosis, implicating the receptor in neurodegeneration as well as in infection, autoimmunity, and cancer immune evasion.

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SKU: 35080491790

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4.1 ★★★★★
Based on 11 reviews
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K
Verified Purchase
Kaitlynne Lynch
Louisville, US
★★★★★ 5
Puppy loves it!
Size: X-Large, Color: Teething Bone, Size: X-Large, Color: Teething Bone
My small puppy loves this bone! I originally purchased this for a larger dog, but my small 10 pound puppy loves it more. It’s good quality and a softer material for puppies!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 10, 2026
J
Verified Purchase
Jesse Arner
Birmingham, US
★★★★★ 4
A hit, but could be stronger
Size: Medium, Color: Ring Bone, Size: Medium, Color: Ring Bone
Gave this to my 11 week old pittie, and she did significant damage within 15 mins. She left a pile of chewed bits on the couch but thankfully didn't eat them. Definitely don't leave unsupervised. That said, she LOVED it and it seemed to help her teething gums so, I'm still giving it a 4/5 Puppy pics for tax
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Reviewed in the United States on June 3, 2026
F
Verified Purchase
FieryFury
Boise, US
★★★★★ 5
My dog loves this toy!!!
Size: Medium (Pack of 1), Style: Stick Medium
I bought this toy for my 80 lb dog who is very rough on her toys. She Loves squeaker toys. This toy held up for about 1 years which is awesome for how aggressive my dog is with her toys. I play tug of war with her with this. The squeaker didn't last too long. Somehow she broke that pretty quickly but the toy itself was very strong and durable. It's a softer rubber like material and it's bendable and twistable. After the toy broke (it actually broke in half), I purchased her another one because of how much she enjoyed it. This newer one, the squeaker still works and have had it for about 6 months or so now. I highly recommend this toy to anyone who has a dog that is an aggressive chewer and who's hard on their toys.
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Reviewed in the United States on April 4, 2025
S
Verified Purchase
Sugarkat
Belleville, US
★★★★★ 5
She goes through things pretty quick, and I know she could have destroyed ...
Size: Large (Pack of 1), Style: Stick Large
My dog is a heavy chewer, and player (Doberman). I got one of these when she was a puppy and about 2 years later it broke in half from periodic chewing and tugging. It was one of her favorites and I was impressed it lasted so long so I ordered another one. She goes through things pretty quick, and I know she could have destroyed this fast as well, but it probably helped that she had other things she was supposed to chew on instead. We never encouraged her to chew this, but she loves bringing it over so we can throw it, or tug on it. Never had any issues with the squeaker (until the first one broke in half after 2 years). I would recommend it, but try to encourage it for play and not for chewing. It is flexible and durable and handles a 70lb Doberman dangling from it periodically.
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Reviewed in the United States on September 19, 2016
G
Verified Purchase
Gen
Pawtucket, US
★★★★★ 4
Fun!
Size: Large (Pack of 1), Style: Stick Large
This was nice and fun for my dogs. Squeegee could have last a little longer. It lasted about 2 weeks until they were bored of it but I think that’s more of a my dog issue.
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Reviewed in the United States on June 10, 2025

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