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Description
Phospho-IRE1 (S724) Recombinant Rabbit mAb (S-3480-15)Product Specification Host Rabbit Antigen Phospho IRE1 (S724) Synonyms Serine threonine protein kinase endoribonuclease IRE1; Endoplasmic reticulum to nucleus signaling 1; Inositol requiring protein 1 (hIRE1p); Ire1 alpha (IRE1a); IRE1; ERN1 Immunogen Synthetic Peptide Location Nucleus Accession O75460 Clone Number S 3480 15 Antibody Type Recombinant mAb Isotype IgG Application WB, ICC Reactivity Hu, Ms Positive Sample Serum starved and Calyculin A
Product Specification
| Host | Rabbit |
| Antigen | Phospho-IRE1 (S724) |
| Synonyms | Serine/threonine-protein kinase/endoribonuclease IRE1; Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1 (hIRE1p); Ire1-alpha (IRE1a); IRE1; ERN1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | O75460 |
| Clone Number | S-3480-15 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms |
| Positive Sample | Serum-starved and Calyculin A treated HeLa, Calyculin A treated NIH/3T3 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms |
| ICC | 1:100 | Hu |
Background
Phospho-IRE1 (S724) refers to the phosphorylated form of inositol-requiring enzyme 1-alpha (IRE1α) at serine residue 724, a critical post-translational modification that serves as a hallmark of IRE1α activation during the unfolded protein response (UPR). As an endoplasmic reticulum (ER) transmembrane protein kinase and endoribonuclease, IRE1α remains in an inactive, monomeric state under basal conditions through binding to the ER chaperone BiP/GRP78; however, upon accumulation of misfolded proteins within the ER lumen, dissociation from BiP triggers oligomerization and trans-autophosphorylation at multiple sites including S724 within its kinase activation loop. This phosphorylation event induces a conformational shift that licenses the RNase domain to excise a 26-nucleotide intron from X-box binding protein 1 (XBP1) mRNA, yielding the potent transcription factor XBP1s that upregulates ER-associated degradation (ERAD) machinery, chaperone expression, and phospholipid synthesis to restore proteostasis. Beyond XBP1 splicing, phospho-IRE1 (S724) also regulates the selective degradation of specific mRNAs and microRNAs through regulated IRE1-dependent decay (RIDD), modulating cellular outcomes ranging from survival to apoptosis depending on stress intensity and duration. Dysregulation of IRE1α phosphorylation at this site has been implicated in diverse pathologies including multiple myeloma, diabetes, neurodegenerative disorders, and inflammatory conditions, making S724-phosphorylated IRE1 a valuable biomarker for ER stress levels and an attractive therapeutic target for small molecule kinase inhibitors aimed at modulating the UPR signaling cascade.
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