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Description
5x Kinase Buffer BProduct Specification Physical Appearance Liquid Stability & Storage For long term storage, keep 5X buffer at 20C. If buffer will be continually used, it is recommended that the 10X buffer be kept at 4C for1 2 weeks. Background Kise Buffer can be used to assay protein kise activity. Components 250 mM Tris, 50 mM CaCl2, 750 mM Cl, 0. 25% (w v) Brij 35, pH 7. 5. Protocol 1. Thaw 5X buffer at 24 30C, mixing end over end. 2. Dilute the 5X buffer to the
Product Specification
| Physical Appearance | Liquid |
| Stability & Storage | For long term storage, keep 5X buffer at -20°C. If buffer will be continually used, it is recommended that the 10X buffer be kept at 4°C for1-2 weeks. |
Background
Kise Buffer can be used to assay protein kise activity.
Components
250 mM Tris, 50 mM CaCl2, 750 mM Cl, 0.25% (w/v) Brij 35, pH 7.5.
Protocol
1. Thaw 5X buffer at 24-30°C, mixing end-over-end.
2. Dilute the 5X buffer to the desired concentration using ddH2O.
Guidelines
For optimal storage, aliquot buffer into smaller quantities after centrifugation and store at recommended temperature. For most favorable
performance, avoid repeated handling and multiple freeze/thaw cycles.
Shipping Notes
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Exchange/Return Notes
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