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Description
Bovine Mammary Glands Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm<(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)<(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion<(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes (2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4 b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A. |
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| Description | Composition:
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| Storage Temp. | 4° C. 3months; -20° C. 1 year, see label for details |
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4.1 ★★★★★
Based on 18 reviews
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Product Reviews
★★★★★ 5
Great
Size: Large, Color: 5 Pack
These are big deal toys for our dogs. Use them when have to go out for a whole day. Have bought three sets over about two years. Takes them a several days to gut and get to the squeaker. They keep the skin around for months if we let them.
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Reviewed in the United States on March 10, 2026
★★★★★ 5
Dogs love them!
Size: Large, Color: 5 Pack
May dogs loved these. The squeaks last long enough but get lots of wear and tear. Worth money for the fun. My dog is 12 and still enjoys perfect size, weight, and flexibility.
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Reviewed in the United States on March 21, 2026
★★★★★ 5
GREAT Value even for a big boy.
Size: Large, Color: 5 Pack
My new 80lb husky/lab rescue LOVED his birthday present. They crinkle has he plays air-fetch w/himself! He does instantly puncture the squeekies at either end, but he's a large dog who chews. And these are SAFE for that. I figure 5 will last 2 months, but for the price? Who cares! He's worth it! Next time I'll go brighter colors because he tosses far &wide.
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Reviewed in the United States on April 22, 2026
★★★★★ 4
Squeaker short-lived, enjoyment long-lasting
Size: Large, Color: 5 Pack
My 10-pound dorkie rips out the squeakers in about 30 minutes. I wish they were sturdier or the parts of the toy housing the squeaker were reinforced. However, he is so excited to get a new one and will continue to play with it even when the squeaker is out. He loves to play tug. They do get pretty gross from the chewing and definitely benefit from a trip through the washer.
While this is not a very sturdy toy it provides so much entertainment I had to give it four stars.
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Reviewed in the United States on March 31, 2026
★★★★★ 5
Pups are happy!
Size: Large, Color: 5 Pack
Our dogs love these toys! What a great find! We will be buying different ones.
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Reviewed in the United States on June 7, 2026