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Description
Human VEGFR-1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Saliva Cell lysis buffer: adherent cells are gently washed with pre-cooled PBS, then digested with trypsin, and collected after centrifugation at 1000×g for 5 minutes; suspended cells can be collected directly by centrifugation. The collected cells are washed 3 times with pre-cooled PBS, and 150-200uL PBS is added to each 1×10^6 cell for re-suspending (it is recommended to add protease inhibitors to PBS; if the content is very low, the PBS volume can be appropriately reduced) and the cells are broken by repeated freezing and thawing or ultrasound. The extract is centrifuged at 2-8°C, 1500×g for 10 minutes, and the supernatant is taken for detection. Follicular fluidOther biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Vascular Endothelial Growth Factor Receptor 1 (VEGFR-1). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Vascular Endothelial Growth Factor Receptor 1 (VEGFR-1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Vascular Endothelial Growth Factor Receptor 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | The transferrin receptor (TFR) is a carrier protein for transferrin. It is required for the import of iron into cells and its expression is regulated by intracellular iron concentration. It imports iron by internalizing the transferrin-iron complex through receptor-mediated endocytosis. Two transferrin receptors, transferrin receptor 1 and transferrin receptor 2, have been characterized. Both are transmembrane glycoproteins. TfR1 is a high-affinity, ubiquitously expressed receptor, while TfR2 expression is restricted to certain cell types and is independent of intracellular iron concentration. TfR2 binds to transferrin with a 25-30-fold lower affinity than TfR1. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, saliva, cell lysate, follicular fluid and other biological fluids |
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4.8 ★★★★★
Based on 23 reviews
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Product Reviews
★★★★★ 5
Smooth taste and blends nicely
Size: 1.4 Ounce (Pack of 1)
This is probably one of the smoothest matchas I've ever bought. The tea blends out nicely without any clumps - no sifting required! I have bought from expensive brands that still have clumps despite sifting and whisking, so I was extremely pleased to see that this one doesn't have any of those problems. The price is also on point!
Taste: 4.5/5 I would say on par if not better than most cafes where you're paying $7-8 a cup. It's not bitter at all and has a mild grassy note. It's tasty on its own or as a latte.
Blendability: 5/5 Doesn't need sifting at all. A bamboo whisk is enough.
Color: 4.5/5 A nice high quality green color. I'd say it's a tier down premium green, but still pretty vibrant and the quality can't be beat for this price point.
Overall thoughts: It's the perfect budget brand if you're looking for a high quality matcha experience. It's great for everyday use and a good starting point for beginners.
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Reviewed in the United States on January 31, 2026
★★★★★ 4
Side-by-Side Comparison of Chinese Matcha on Amazon
Size: 1.4 Ounce (Pack of 1), Size: 1.4 Ounce (Pack of 1)
A few weeks ago, I decided to purchase several of the top-selling Chinese matcha products on Amazon to conduct a side-by-side comparison. My intention was to test them under the same conditions and share my honest experience for anyone considering these products.
**A Little Background About Me**
As a daily matcha drinker, I consider myself somewhat of a matcha snob. I've tried various mid- to high-end Japanese brands and a few Western-Japanese ones, mainly from Amazon. I enjoy my matcha without sweeteners, typically having a latte and an usucha each day, with an occasional koicha if the quality is good. This routine gives me a sensitive palate, which might bias my reviews.
Given the current matcha shortage in Japan, I wanted to explore Chinese matcha available on Amazon for daily lattes, though I wasn't looking for something for usucha. After testing nine different Chinese matcha products, I found none suitable for my taste. However, beginners or those who enjoy sweetened drinks might appreciate some of these options.
I have tried higher-quality Chinese matcha from other sources that I would consider for lattes and even usucha, but those aren't typically found on Amazon, similar to high-end Japanese matcha.
**How I Evaluated These Matchas**
My ratings are based on the following criteria:
- Color (dry powder and prepared)
- Texture and fineness
- Aroma
- Whisking and microfoam quality
- Mouthfeel
- Bitterness and astringency
- Overall balance and drinkability
👉 These ratings are comparative only among the Chinese matcha products sold on Amazon, not against Japanese matcha as a whole.
**A Quick Note on Flavor Descriptors**
You’ll notice that I frequently mention “grassy,” so it’s important to clarify what I mean by that.
In this context, a grassy note in matcha is different from the vegetal characteristics commonly referenced when describing matcha's flavor.
For me, vegetal notes can feel fresh, green, and clean, whereas grassy notes refer to a muddy, astringent, sometimes dirt-like mouthfeel, especially when unbalanced.
Some Japanese matchas may have grassy notes, but they are generally balanced with other flavor profiles like umami, nuttiness, and creaminess. In many of the products I tested here, grassiness dominated the flavor, and I couldn't detect much umami or other tastes.
It's also worth mentioning that many of these matchas seem to originate from the same region and possibly the same factories. Some may be white-label products with different quality standards from the same factory.
I can confidently say that none of these matchas were true stone-milled matcha, regardless of what the sellers advertised. The powders were generally coarser and grainier than high-quality Japanese matcha.
Lastly, please note that the photos may not accurately reflect the true color of the matcha due to lighting and oversaturated imaging from my iPhone.
# Individual Rankings (From Worst to Best)
**9. MatchaAdmire**
⭐ 1 / 5
This was the most difficult matcha for me to drink. The powder had a noticeably brownish tone, with very high bitterness and astringency, resulting in a muddy mouthfeel. Even when used as a latte base, it felt harsh and unbalanced. I wasn’t able to finish the cup.
**8. Efoofan**
⭐ 1 / 5
This matcha had the weakest color in the group and a very coarse texture. While it was slightly less harsh than the previous entry, it remained highly astringent, muddy, and grassy. I found it unenjoyable without heavy masking.
**7. VenturePal**
⭐ 1.5 / 5
VenturePal had better color and slightly improved frothing compared to the two below it, but bitterness and astringency were still high. The texture remained coarse, and overall balance was lacking.
**6. MatchAiA – Ceremonial Grade**
⭐ 2 / 5
The color was a few shades better than the lower-ranked products, and bitterness was reduced, but the powder was still grainy and strongly grassy with noticeable astringency. This seems to be a white-label product from the same source as Emerail, but at a lower quality tier.
**5. CHAYEAH**
⭐ 2.5 / 5
This was a noticeable step up. The color improved, and it finally smelled like actual matcha. However, grassy green tea notes still dominated the flavor. I could see this being enjoyable for beginners or those who prefer sweeteners, but the marketing claims about usucha or koicha seem unrealistic at this quality level.
**4. Chaism – Ceremonial Grade (Single Origin)**
⭐ 2.5 / 5
Chaism had promising color and excellent frothing. The first few sips were acceptable, but the aftertaste became muddy and unpleasant for me. This may come down to personal preference, but despite its better appearance, the finish was not enjoyable.
**3. Chaism Premium Harvest**
⭐ 3 / 5
This was an improvement over the Single Origin version, with better balance, less bitterness, and a more approachable flavor overall. It remained grassy but was less aggressive, making it workable for daily lattes with moderate sweetener.
**2. Emerail Matcha**
⭐ 4 / 5
Emerail was one of the strongest performers in this comparison. The powder felt fresher, had a cleaner aroma, and exhibited lower bitterness and astringency. It whisked easily with water, creating a pleasant matcha experience.good microfoam.
Still grassy, but better controlled and smoother than most competitors. It’s clear this brand keeps the better material for its own label.
** 1. Jiuyu Matcha**
⭐ 4 / 5
The best overall performer among the Chinese matcha I tested on Amazon.
It had the best color, a strong and pleasant green-tea aroma, smoother texture, and lower bitterness. While slightly grassier than Emerail, it was less grainy and had a better overall mouthfeel.
Final Thoughts
I wouldn't use any of these matchas for my daily latte. That said, this doesn’t mean they won’t work for others. These matchas can be suitable if:
1- You’re new to matcha
2- You primarily drink matcha lattes with syrups
3- And you want an affordable daily option
Lastly, I wanted to mention that I’ve had excellent Chinese matcha outside of Amazon, including some I would happily drink daily and even use for usucha. Based on this comparison, those higher-end options simply aren’t what’s commonly available on Amazon right just as is the case for well-known Japanese-owned brands.
This review reflects my personal palate and preparation style, and I hope it helps others choose a product that best fits how they enjoy matcha.
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Reviewed in the United States on December 18, 2025
★★★★★ 5
smooth and great quality matcha
Size: 1.4 Ounce (Pack of 1), Size: 1.4 Ounce (Pack of 1)
I was surprised by how fresh and vibrant this matcha is! The color is a beautiful bright green, and it blends really smoothly — no clumps at all. The flavor is rich but not very bitter, perfectly match with coconut water. I’ve been using it for lattes and coconut water, and it tastes amazing either way. You can really tell it’s high-quality matcha. Definitely will repurchase once I finish this bottle!
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Reviewed in the United States on November 7, 2025
★★★★★ 5
Great Matcha for Price! 🍵
Size: 1.4 Ounce (Pack of 1), Size: 1.4 Ounce (Pack of 1)
I was hesitant at first because the price seemed too good to be true, but this turned out to be amazing for the cost! It’s not the brightest “ceremonial first harvest” matcha I’ve tried, but it’s also not dull or too yellow tinted. The taste and aroma are both nice, and it works perfectly in lattes or on its own. It whisks up silky smooth. You get a great amount for the price too, some brands charge $20+ for a similar amount and quality. Overall, an amazing affordable find for matcha lovers!
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Reviewed in the United States on March 27, 2026
★★★★★ 5
So good!
Size: 1.4 Ounce (Pack of 1)
My favorite ceremonial grade matcha powder. It's so good and rich. Easy to mix.
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Reviewed in the United States on April 21, 2026