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Description
Caspase 4 Activity Detection KitProduct Specification Usage Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12. 5, 6. 25, 3. 125 M. Set the zero tube without pNA. 2. Take 100l of each concentration and add it to a 96 well plate or 100l cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x axis, and the corresponding pNA concentration is the y axis.
Product Specification
| Usage | Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12.5, 6.25, 3.125 μM. Set the zero tube without pNA. 2. Take 100μl of each concentration and add it to a 96-well plate or 100μl cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x-axis, and the corresponding pNA concentration is the y-axis. Use Excel to make a standard curve of pNA concentration versus OD405 value. Detection of Caspase 4 enzyme activity: 1. Take out an appropriate amount of Ac-LEVD-pNA (2mM) and place it on an ice bath for later use. 2. According to the 100ul reaction system: blank control group: 40μl of detection buffer, 0μl of sample to be tested, 50μl of lysate, 10μl of Ac-LEVD-pNA (2mM); Sample group: 40μl of detection buffer, 50μl of sample to be tested, 0μl of lysate, 10μl3 of Ac-LEVD-pNA (2mM). Add Ac-LEVD-pNA (2mM) and mix well. Be careful to avoid bubbles during mixing. Incubate at 37 º C for 60-120 minutes. A405 can be measured when the color change is obvious. If the color change is not obvious, the incubation time can be appropriately extended, or even overnight. 4. The A405 of the sample minus the A405 of the blank control is the absorbance produced by pNA catalyzed by caspase 4 in the sample. By comparing the standard curves obtained in the above steps, it is possible to calculate the amount of pNA catalytically produced in the sample. |
| Theory | Based on Caspase 4, it can catalyze the substrate Leu Glu Val Asp p nitroanilide (Ac LEVD pNA) to produce yellow pNA (p-nitroaniline), which can be used to detect the activity of Caspase 4 by measuring absorbance. pNA has a maximum absorbance at 405 nm. Caspase activity was obtained by measuring pNA using visible light colorimetry. Suitable for detecting Caspase 4 activity in mammalian tissues and cells. |
| Experiment Reagent Instrument | Microplate reader or spectrophotometer |
| Synonym | caspase-4,caspase 4 |
| Description | caspase 4 Activity Detection Kit is a kit that detects caspase 4 enzyme activity or purified caspase 4 enzyme activity in cell or tissue lysates by spectrophotometry. |
| Storage Temp. | When stored at-20 °C, Ac-LEVD-pNA and pNA need to be stored in the dark. |
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★★★★★ 5
Bello brillo corporal
Color: Silver, Color: Silver
Tu cuerpo queda con un brillo espectacular pareces modelo
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Reviewed in the United States on May 22, 2026
★★★★★ 5
shimmer
Color: Rose Gold
I recently tried the UCANBE Body Shimmer Oil, and I have to say, I'm really impressed! The oil has a beautiful, subtle shimmer that adds a touch of glow without being too overpowering. It feels luxurious on the skin, absorbing quickly and leaving it soft and smooth. I love how it gives my skin a healthy, radiant look, especially when I wear it on my legs and arms. The scent is light and refreshing, not too strong, which is a plus for me. Overall, it's perfect for a little extra sparkle on special occasions or just when I want to feel a bit extra on a regular day! Definitely recommend it if you're looking for a quality shimmer oil that doesn't feel greasy.
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Reviewed in the United States on April 1, 2025
★★★★★ 1
Bad Batch
Color: Gold
It was so thick & stinky. Had to toss it and take a shower right away. Couldn’t get it off my skin.
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Reviewed in the United States on February 28, 2026
★★★★★ 5
INDESTRUCTIBLE!
Our lab chews through every “indestructible” toy within a few hours. This ball is amazing! He has not been able to destroy this toy! Highly recommend for your most aggressive chewer!
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Reviewed in the United States on March 26, 2026
★★★★★ 5
Our dog is very happy with this ball!
Our lab is an aggressive chewer and LOVES his toys. I was looking for a chew toy that had a different shape than a regular ball or Kong and saw this. Our dog LOVES this toy because he can chew it when he wants to be alone and it keeps him entertained (especially when stuffed with food). He also plays "catch" with us by rolling the ball toward us with his nose. So far there is no indication that he is damaging it so I'm very pleased with the durability.
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Reviewed in the United States on May 20, 2026