SKU: 50423812708

Human IL-12/IL-23 p40 ELISA Kit

Sale price$202.50 Regular price$225.00
Save 10%

Pay in installments of $56.25 with ShopPay, AfterPay and Klarna

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Aug 25 - Aug 30

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

Human IL-12/IL-23 p40 ELISA KitProduct Specification Species Reactivity Human Synonym Human IL 12 ELISA kit, IL 23 p19 IL 12 p40, IL23, IL 23, IL 23A, IL 23 A, IL 23p19, IL 23p19 IL 12p40, IL23P19P19, interleukin 23 p19 subunit, interleukin 23, alpha subunit p19, interleukin 23 subunit alpha, Interleukin 23 subunit p19, JKA3 induced upon T cell activation, MGC79388, SGRF, SGRFIL 23 subunit alpha, Description Detection principle: Double antibody sandwich ELISA was used in this

Product Specification

Species Reactivity Human
Synonym Human IL-12 ELISA kit, IL-23 p19/IL-12 p40, IL23, IL-23, IL-23A, IL-23-A, IL-23p19, IL-23p19/IL-12p40, IL23P19P19, interleukin 23 p19 subunit, interleukin 23, alpha subunit p19, interleukin-23 subunit alpha, Interleukin-23 subunit p19, JKA3 induced upon T-cell activation, MGC79388, SGRF, SGRFIL-23 subunit alpha,
Description

Detection principle:

Double antibody sandwich ELISA was used in this experiment. Anti-human IL-12/IL-23p40 monoclonal antibody is coated on a microplate, and IL-12/IL-23p40 in samples and standards will bind to the antibody immobilized on the plate, and the free components will be washed away; Horseradish peroxidase-labeled anti-human IL-12/IL-23 p40 polyantibody was added and unbound antibody was washed away; Adding a substrate solution (chromogenic agent), the color of the solution is proportional to the bound target protein; A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).

Test Type:Double antibody sandwich method

Form:Pre-coated 96-well plate

Test Sample Type:Cell supernatant, serum, plasma

Sample Load:100ul

Kit Components:

A copy of the pre-coated 96-well plate, standard, anti-human IL-12/IL-23 p40 detection antibody, buffer for dilution, chromogenic solution (A, B), wash solution, stop solution, SA-HRP, plate sealing membrane, and instructions.

Sensitivity:3.5 pg/mL

Detection range:62.5-4,000 pg/mL

Recovery Range:89-114%

Save Method:2-8℃

Standard curve plot

Background:

IL-12, also known as natural killer cell stimulating factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine mainly produced by antigen-presenting cells (monocytes/macrophages, dendritic cells, and B lymphocytes). IL-12 has a variety of effects on T lymphocytes and natural killer (NK) cells, including the ability to stimulate cytotoxicity, proliferation, cytokine production, and Th1 subset development.

IL-12 is a 70 kDa (p70) heterodimeric glycoprotein linked by disulfide bonds, consisting of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The p40 and p35 subbases do not possess IL-12 activity in themselves, but homodimers of p40 have been shown to bind IL-12 receptors and are IL-12 antagonists.

The genes of human p40 and p35 located on chromosomes 5 and 3, respectively, are independently regulated. The expression of p35 mRNA has been found to be almost ubiquitous, however, the p35 subunit was not detected in the culture supernatant of cells expressing p35 or p35 and p40 mRNA only. The p40 mRNA expression level was higher in cells expressing both p35 and p40 mRNA, and together with the heterodimeric IL-12 p70, a free p40 subunit unrelated to the p35 subunit was secreted. Most of the free p40 subunits secreted by the various human cell lines examined have been found to exist in monomeric form. The content of free p40 far exceeds p70 in the supernatants of various activated human monocytes, and the levels of p70 measured by bioassays are consistent with those measured using p70-specific immunoassays, suggesting that p40 monomers are not potent IL-12 antagonists. Currently, the physiological role of the free p40 subunit is unclear.

 

Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 50423812708

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.1 ★★★★★
Based on 17 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
D
Verified Purchase
Dr. DeeJaye
Port Orchard, US
★★★★★ 5
Just what I wanted.
Color: Black, Navy Blue, Wine Red, Size: 5X-Large
These may have just become my standard shirt to wear. Feels good whether tucked or out. Drapes well and fits well. So far, I can see it adjust to the different undershirt I may wear. Will probably order another set in other colors and a back up set of the basics because they will get worn a lot.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 17, 2025
C
Chloe
Belleville, US
★★★★★ 3
Its alright
Color: Black, Size: 5X-Large
True to size per the size chart. The fabric is soft but it it feels cheap to me. It attracts lint but you can brush it off easily so its not the cheapest material I've dealt with. Also, it is opaque (not see through). There is no front/ back indicator either which is indicative of a cheap shirt in my opinion. Would be friendly for tag sensitive people (to stay positive).
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 14, 2026
A
Verified Purchase
Amazon Customer
Charlottesville, US
★★★★★ 5
Fit and colors are perfect
Color: Black, White, Apricot, Size: Large
They fit very well, I got them for my husband and he was very excited for the style & fit i am actually going to buy him another pack
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 7, 2025
C
Verified Purchase
ChilledOut
Alexandria, US
★★★★★ 1
Fuzzballs get wearing once
Color: Black, Size: X-Large
Very smart to wear, but the quality is terrible so after one wash the collar start fraying and fuzzballs start forming. Very disappointing.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 2, 2026
J
Verified Purchase
Jimmy
San Leandro, US
★★★★★ 5
Takes a Licking and Keeps on Ticking
Color: Green/Black
I love this watch despite the fact that it is really a toy. I love the look of the watch, I love how lite the weight of the watch is on my wrist, and it really does keep good time if you manage the solar charge properly. Other than looking cool on your wrist, this watch has absolutely no functions other than time keeping. The black plastic bezel is molded into the case and does not turn, so don't plan on using that. The watch has no light, the dial numbers are non-luminous and the hands are very limited in terms of luminosity. No buttons. All you can do is adjust the time and date by pulling out what used to be a winding stem in the good old days. The small tag on the strap with Geographic coordinates shown on it indicate the location of the old Timex manufacturing facility (SW of Waterbury, CT). I have a nice ledge at my bedroom window and it gets good western sun for a good part of the day regardless of the time of year. On this ledge you will find a half dozen solar powered watches (mostly Casio) properly aligned to maximize the charge they may receive each day. Note that the description for this Timex watch specifically states that a lightbulb won't charge this watch. You need direct sunlight for several hours if you want to get more than a 3-week charge into this watch. So, I'll leave you with a couple of tips, my friends; 1.) If you are looking for a first watch to give your outdoorsy oriented Son or Daughter, then give them this one. Why? Because it is cool looking, fairly well built, and it actually works, 2.) After you receive your Timex watch, be sure to keep the display mount it is packaged with. It makes a great fixture to mount your watch on for charging and allows you to maximize the position of the solar cells on the dial so that you can optimize charging time. Have Fun! Jimmy
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 28, 2026

recommand products