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Description
Alexa Fluor® 647 Mouse Anti-Human CD73 Antibody (S-715-115)Product Specification Host Mouse Antigen CD73 Synonyms 5' nucleotidase, 5' NT, 5' deoxynucleotidase, Ecto 5' nucleotidase, IMP specific 5' nucleotidase, Thymidylate 5' phosphatase, NT5E, NT5, NTE Immunogen Recombinant Protein Location Cell membrane Accession P21589 Clone Number S 715 115 Antibody Type Mouse mAb Isotype IgG2b,k Application FCM Reactivity Hu Purification Protein A Concentration 0. 05mg ml Conjugation Alexa Fluor 647 Physical Appearance
Product Specification
| Host | Mouse |
| Antigen | CD73 |
| Synonyms | 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, Thymidylate 5'-phosphatase, NT5E, NT5, NTE |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P21589 |
| Clone Number | S-715-115 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.05mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume |
Background
NT5E (CD73) is a surface enzyme which is expressed on multiple cells. This enzyme mediates the gradual hydrolysis of the autocrine and paracrine danger signals of ATP and ADP to anti-inflammatory adenosine. Immune suppression mediated by adenosinergic pathways is very important for maintaining immune system homeostasis. Immune suppressive functions of T regulatory cells are also dependent on CD73 expression. CD73 also occurs on anergic CD4 + T cells, thereby maintaining self-tolerance to healthy tissues as well as protecting the fetus from the mother's immune system during pregnancy.
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Exchange/Return Notes
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