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Description
Pig Endometrial Carcinoma Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm<(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)<(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion<(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4  & Nbsp& Nbsp& Nbsp& Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A added. |
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| Description | Domposition:
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| Storage Temp. | 4° C. 3months; -20° C. 1 year, see label for details |
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4.3 ★★★★★
Based on 21 reviews
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Product Reviews
★★★★★ 5
Very cute, good quality
Style: Lambchop, Size: 10", Style: Lambchop, Size: 10"
Our Two year-old Great Pyrenees rescue loves her Lambchop. She’s fairly gentle with it, but it did accidentally rip so we had replace it since she loves it so much. No fault of the product. The product is very well made, She just got a little rough with it.
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Reviewed in the United States on April 27, 2026
★★★★★ 5
We <3 big lambchops
Style: White, Size: 24" Jumbo, Style: White, Size: 24" Jumbo
These are my vizsla's absolute FAVORITE toy. I mean, she doesn't leave the room without bringing at least one. We've bought several of these exact toys now and they're surprisingly durable- we've even put a few through the laundry. She loves to snuggle, nibble, and carry this toy like a security blanket. These are the big ones, so they get a little discolored from her grabbing them around the belly. The squeakers are pretty few and far between so it's generally not noisy unless she's super bored or excited. Get it, your dog will love it and they're cheaper than some of the small ones.
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Reviewed in the United States on March 7, 2026
★★★★★ 5
A Classic Toy My Dog Can’t Get Enough Of!
Style: Blue Birthday Hat, Size: 10.5"
The Multipet Lamb Chop Dog Plush Toy (10.5”, Cream) is an absolute winner in our house! This adorable, nostalgic plush is just as cute as it is entertaining, and my dog fell in love with it the moment it came out of the package.
Super Soft & Cuddly
The material is extra plush and velvety, making it perfect for dogs who love to snuggle. It feels well-made and not scratchy like some cheaper toys.
Squeakers That Keep Dogs Engaged
There are multiple squeakers inside, and they’re loud enough to keep your dog excited but not obnoxious. My pup carries it around proudly and squeaks it nonstop.
Great Size
The 10.5” size is ideal for both small and medium to large dogs. Big enough to cuddle, small enough to toss around easily.
Durable for a Plush Toy
While it’s not built for heavy destroyers (no plush toy truly is), it holds up surprisingly well to daily play and lots of shaking.
Overall:
The Multipet Lamb Chop is a fantastic combo of cuddly plush and squeaky fun. Perfect for fetch, snuggles, or as your dog’s new favorite companion. If your dog enjoys plush toys, this is definitely one to grab.
Highly recommended! 🐑🐶💛
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Reviewed in the United States on December 11, 2025
★★★★★ 5
BEST TOY!
Style: White, Size: 6"
I recently bought the Multipet INTERNATIONAL 843140 Lambchop Plush Squeak Toy Mini for my small dog, and it has quickly become her favorite toy. Here’s why this little squeak toy is a great addition to any pet’s collection:
Adorable Design
The Lambchop Plush Squeak Toy is absolutely adorable. It resembles the beloved character Lambchop, which adds a nostalgic charm for those who remember the classic TV show. The toy is well-crafted with a cute, friendly face and soft, plush material. Its small size makes it perfect for petite pets, and it’s just the right size for my dog to carry around and snuggle with.
Engaging and Fun
The built-in squeaker adds an extra layer of excitement for my pet. Every time she bites down, the squeak captures her attention and keeps her engaged for longer periods. It’s great for interactive play, fetch, and even as a comforting chew toy. My dog loves the noise it makes and often brings it to me to initiate playtime.
Durable Construction
Despite its soft and plush exterior, the Lambchop toy has proven to be quite durable. My dog is a moderate chewer, and after several weeks of play, the toy is still intact with no signs of wear and tear. The stitching is strong and has held up well against my dog’s playful bites and tugs. However, for very aggressive chewers, it might not last as long.
Easy to Clean
The toy is also relatively easy to clean. I’ve hand-washed it a few times to keep it fresh, and it dries quickly without losing its shape or squeak. The white color, while prone to getting dirty, can be easily spot-cleaned to maintain its appearance.
Perfect Size for Small Pets
At 6 inches, this toy is ideally sized for small dogs and even cats. It’s lightweight and easy for them to carry around. My dog can comfortably grip it in her mouth, and the toy is soft enough for her to cuddle with during nap time.
Value for Money
Considering its durability, engaging design, and the joy it brings to my pet, the Multipet Lambchop Plush Squeak Toy offers excellent value for money. It’s reasonably priced and provides hours of entertainment and comfort for my dog.
Overall Impression
Overall, the Multipet INTERNATIONAL 843140 Lambchop Plush Squeak Toy Mini is a fantastic toy for small pets. Its adorable design, engaging squeaker, and durable construction make it a worthwhile purchase. My dog loves it, and it has quickly become a staple in her toy collection. I highly recommend this toy to anyone looking for a cute and fun squeak toy for their small pet.
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Reviewed in the United States on June 18, 2024
★★★★★ 4
Great, but a little big…
Style: Lambchop, Size: 10"
Super soft and cute, I got this for my toy Cavapoo puppy. The different textures are perfect. It is a little bigger than I was expecting, and will probably be about the same size as my puppy when I get her, but I’m sure she’ll love it. I don’t know how safe it will be yet since I’ve yet to be able to watch her play with it, but I don’t see anywhere that could have loose strings for her to choke on, so I’d say it’s pretty safe. Overall a perfect toy for dogs, with a squeaker and nice textures for the dog to chew on.
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Reviewed in the United States on May 4, 2026