Human IgG ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA detection technology. Specific anti human IgG capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the microplate plate for incubation. After incubation, the IgG present in the sample binds to the solid-phase antibody. After washing to remove unbound material, horseradish peroxidase (HRP) - labeled detection antibody was added for incubation. After washing, the chromogenic substrate TMB was added to avoid light for color development. The depth of color reaction is directly proportional to the concentration of IgG in the sample. The reaction was stopped by adding stop solution, and the absorbance value was measured at 450 nm wavelength (correction wavelength 570 - 630 nm).

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, HRP labeled IgG detection antibody, detection buffer, TMB chromogenic substrate, washing solution, termination solution, plate sealing membrane and instructions.

Sensitivity: 0.18 ng/ml

Detection Range: 3.13-200 ng/ml

Recovery Range: 93-110%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Immunoglobulin G (IgG)Is an antibody, which is a protein complex composed of four peptide chains &mdash&Mdash; A Y-type antibody monomer consisting of two identical heavy chains and two identical light chains. Each IgG has 2 antigen binding sites. IgG is the main antibody of blood and extracellular fluid, which can control the infection of human tissues. Plasma B cells can produce and release IgG molecules.

IgG is the only immunoglobulin that can pass through the human placenta, thus providing protection for the fetus in utero. IgG antibodies extracted from the plasma of blood donors can be used as intravenous immunoglobulins to treat immunodeficiency, autoimmune diseases and infectious diseases.