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Description
Annexin V-APC/7-AAD apoptosis analysis kitProduct Specification Usage 1. Instrument parameter adjustment 1. Collect 1 106 3106The cells were washed twice by centrifugation with pre cooled PBS and the supernatant was discarded. 2. Add 500uL of Apoptosis Positive Control Solution to resuspend and incubate on ice for 30 minutes. 3. Wash by centrifugation with pre cooled PBS and discard the supernatant. 4. Add an appropriate amount of pre cooled 1 Binding Buffer to resuspend, and add the same
Product Specification
| Usage | 1. Instrument parameter adjustment 1. Collect 1 × 106-3×106The cells were washed twice by centrifugation with pre-cooled PBS and the supernatant was discarded. 2. Add 500uL of Apoptosis Positive Control Solution to resuspend and incubate on ice for 30 minutes. 3. Wash by centrifugation with pre-cooled PBS and discard the supernatant. 4. Add an appropriate amount of pre-cooled 1 × Binding Buffer to resuspend, and add the same number of untreated viable cells to mix with it. Add pre-cooled 1 × Binding Buffer to replenish to 1.5 mL, and divide it into three tubes equally, of which one tube is a blank control tube and two tubes are single dye tubes. 5. Add 5 uL of Annexin V-APC or 10 uL of 7-AAD to the single stain tube and incubate for 5 minutes at room temperature and protect from light. 6. On the flow cytometer, the voltages of FSC, SSC and fluorescence channel were adjusted with a blank tube, and under this voltage condition, the fluorescence was adjusted with a single staining tube Compensation of channels. Note:Certain processes of treating adherent cells as single cells cause cell membrane damage, resulting in false positives for Annexin V. Therefore, optimization is needed. It is recommended to treat adherent cells with enzymes that are more gentle on cells, such as Accutase. 2. Sample testing 1. Induce apoptosis according to the experimental protocol. 2. Centrifuge washing with pre-cooled PBS and collect 1-10 × 105Cells (including cells in the culture supernatant). Dilute 5 × Binding Buffer into 1 × working solution with double distilled water, and take 500 uL of 1 × Binding Buffer to resuspend the cells. 3. Add 5 uL of Annexin V-APC and 10 uL of 7-AAD per tube. 4. After gently vortex and mix well, incubate at room temperature and protect from light for 5 minutes. 5. Get on the computer for analysis. |
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| Beilstein | 0 | |||||||||||||||
| Synonym | Annexin V-APC/7-AAD Apoptosis Analysis Kit | |||||||||||||||
| Description |
Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is present in the inner lining of the plasma membrane of normal cells, but in the early stage of apoptosis, membrane asymmetry is lost and PS translocates to the cell surface. Fluorescently Labeled Annexin V Kit Components:
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| PubChem CID | 0 | |||||||||||||||
| General Notes | 1. Please read the instructions carefully before using this product. This product is for scientific research only, not diagnostic. 2. For your safety and health, please wear experimental protective clothing, gloves, masks and other necessary protective equipment. |
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| Storage Temp. | Store at 2-8 °C, do not freeze, shelf life 1 year. |
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4.6 ★★★★★
Based on 21 reviews
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Product Reviews
★★★★★ 4
Can opener
It doesn't shut off by itself when done and doesn't shut off when I push the button, so after a while it will shut off but when it wants too after you take it off the can ,like 30 seconds. But I do like the way it cuts, a clean cut and lid removal nice.
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Reviewed in the United States on February 2, 2026
★★★★★ 5
terrible item
it worked a few times and it doesnt work at all! Terrible item!
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Reviewed in the United States on June 7, 2026
★★★★★ 1
Very dangerous metal shavings!!!
Negative stars if I could.
This is a very dangerous product. It worked well for a while. But then it quit effectively removing the lid instead I got little tiny shavings of metal around the edge. These shavings of metal go into the food and very dangerous.
So this was a waste of money and incredibly dangerous. I am throwing it in the trash today.
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Reviewed in the United States on March 25, 2026
★★★★★ 5
Practical and easy to use
This electric can opener performs its function well and gets the job done effortlessly. With just the press of a button, it opens cans quickly and smoothly—which is very useful, especially if you prefer not to exert physical force. The design is comfortable to handle and does not take up much space in the kitchen. The battery life is good, and it recharges without any issues. Overall, it is a functional product that makes an everyday chore much easier.
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Reviewed in the United States on April 20, 2026
★★★★★ 5
Works great
Ive broken so many manual can openers from sheer strength and this eliminates that issue not only because it no longer needs my input but because this device actually works! Would definitely recommend it. Usb c charging is a god send, no batteries!
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Reviewed in the United States on April 20, 2026
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