Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Sep 1 - Sep 6
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human MGL ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against lipase and monoacylglycerol (MGL). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of lipase and monoacylglycerol (MGL) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Lipase, Monoacylglycerol ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Monoacylglycerol lipase (MGL), also known as MAG lipase, acylglycerol lipase, MAGL, MGL, or MGLL, is an enzyme encoded by the MGLL gene. It is a 33 kDa, membrane-associated member of the serine hydrolase superfamily and contains the classic GXSXG consensus sequence common to most serine hydrolases. The catalytic triad has been identified as Ser122, His269, and Asp239. It catalyzes a chemical reaction that utilizes water molecules to break down monoglycerides of long-chain fatty acids. It works alongside hormone-sensitive lipase (LIPE) to hydrolyze triglyceride stores within adipocytes and other cells into fatty acids and glycerol. It also complements lipoprotein lipase (LPL) to complete the hydrolysis of monoglycerides produced by the degradation of lipoprotein triglycerides. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.1 ★★★★★
Based on 29 reviews
Sort
Product Reviews
★★★★★ 5
Incredibly Comfortable and Supportive — My New Favorite Pillow！
I wasn’t expecting to love this pillow as much as I do, but the DIORIS large sleeping pillow really exceeded my expectations. From the first night, I noticed a difference in the way my neck and shoulders felt in the morning—less tension, better alignment, and overall more restful sleep.
The fill is just the right balance between plush and supportive. It doesn’t flatten out during the night, which is a problem I’ve had with other pillows, especially those that come overstuffed or too soft. This one holds its shape well but still has that cozy, cloud-like feel when you lay your head on it.
I’m a side and back sleeper, and this pillow works beautifully in both positions. It’s also large enough that I don’t feel like I’m falling off it during the night, but not so big that it takes over the whole bed.
One suggestion: if you’re picky about loft, you might want to give it a gentle fluff or adjust the fill before your first night. But honestly, I didn’t need to do much—it was pretty much perfect out of the bag.
Highly recommended if you're looking for a high-quality, comfortable, and generously sized pillow that supports a great night’s sleep!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 9, 2025
★★★★★ 5
Hotel like pillow
These pillows were a pleasant surprise. I bought the set for our guest room but ended up keeping them for our own bed after trying them out. They feel incredibly soft yet still provide enough support to keep my neck from feeling stiff in the morning. I sleep on my side and back, and these work great for both positions. My spouse, who usually runs hot at night, said they stayed cool and breathable.
The material feels high quality, and I love that they’re machine washable without losing their shape. They fluffed right back up after drying. If you're looking for that plush hotel-pillow feel with everyday practicality, this set really delivers.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 15, 2025
★★★★★ 4
Very soft, not firm at all
This pillow is very floppy and not firm at all. It is quite large and I was surprised at the size. My dog has taken a liking to it and decided it's now her new dig bed, which is fine by me, because it's really too soft for my liking. If you like soft pillows then this is for you. If you require something with a bit more support then you won't enjoy this pillow.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 11, 2025
★★★★★ 5
Soft and supportive
I recently purchased the this pillow and I am happy with the quality and comfort it provides. Pillow is soft, plush, and supportive without being too firm. The down alternative fill offers the balance of fluffiness and structure, making them ideal for all sleep positions. Whether I’m sleeping on my back, side, or stomach, these pillows adapt beautifully and keep me comfortable throughout the night. They’re also breathable and skin-friendly, which is a huge plus for anyone with sensitive skin. After using them, I wake up feeling well-rested and free of neck stiffness. I like it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 22, 2025
★★★★★ 5
Pillow
These DIORIS pillows are a game-changer! I’ve tried many pillows over the years, but these are by far the best. The down alternative fill is incredibly soft yet supportive, providing just the right amount of fluffiness without feeling too firm or too soft. I sleep on my side, and these pillows cradle my head and neck perfectly, giving me the support I need for a restful night. They’re breathable and skin-friendly, which is great since I tend to get hot while I sleep. The queen size is just right for my bed, and the pillows maintain their shape night after night. They feel like luxury hotel pillows! Highly recommend for anyone looking for a comfortable, supportive, and durable pillow.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 20, 2025
recommand products
Magura 7.S Disc Brake Pads - Sport Compound Steel Backing For 2 Piston Calipers
26.00
HellermannTyton TSRP3W-36 End Cap, 1-3/4", PVC, White, 1/bag
27.37
Magura Alloy Handlebar Clamp Chrome
17.00
Spank Spoon 2 Stem - 31.8mm Clamp - 40mm Length - Green
36.00
HellermannTyton TSRP2W-36 End Cap, 1-1/4", PVC, White, 1/bag
25.06