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Description
Mouse Liver Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) the tissue after sampling must be placed in the pre cooled (2 8 c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information (2) prepare several Petri dishes and add 4 pre cooled primary culture buffer B for standby (3) disinfect the sampling bottle, put the tissue into the Petri dish,
Product Specification
| Usage |
1. Primary (1) the tissue after sampling must be placed in the pre cooled (2-8° c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information (2) prepare several Petri dishes and add 4 ℃ pre cooled primary culture buffer B for standby (3) disinfect the sampling bottle, put the tissue into the Petri dish, wash it three times with primary culture buffer B, remove impurities, and cut the tissue into a tissue block with a volume of about 1-3mm3by using ophthalmic scissors or scalpel (4) the tissue was digested with the digestive solution c of primary mouse normal liver tissue, and then it was shaken at 37 ℃ for 10-20min (the digestion condition was observed at any time during the digestion process) (5) take a small amount of liquid and observe it under the microscope. After observing more single cells or cell clusters below 70UM under the microscope, add three times the volume of primary culture buffer B to stop digestion (6) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation (7) Matrigel calculation: after step 6, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate (8) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃) (9) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a gel, and add mouse normal liver organoid medium a (restore room temperature) for culture 2. Organoid subculture (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4  &Nbsp&Nbsp&Nbsp b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4 (4) after organoids were collected, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul of mouse normal liver organoid culture medium a 3. Organoid cryopreservation (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid (4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube (5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage 4. Organoid resuscitation (1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube (2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting (3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min (4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g (5) resuspend Matrigel, lay 25ul Matrigel per well in a 24 well cell culture plate, place in an incubator for 10-15min to form a gel, and add 500ul of mouse normal liver organoid culture medium A. |
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| Description |
Composition:
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| Storage Temp. | 4° C. 3months; -20° C. 1 year, see label for details |
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★★★★★ 5
These pillows perform wonderfully and they hold up!
Color: Grey, Size: Standard, Color: Grey, Size: Standard
I have had these pillows for 5 years, so mine is not in brand new condition in this photo but they still appear pretty good and function wonderfully. I recommend these to others looking for a soft but supportive pillow. They are the standard microfiber cooling ones. I have one and my daughter (7) has the other. We use them every night and they are just the perfect amount of medium-softness for both of us despite us being very different in size! Still fluff back up, have just enough support and height or thickness in my opinion (I don’t like very firm microfiber pillows as much, and I don’t like the feather pillows that aren’t goose down because they get crunchy and flat). Love these pillows, the price is super reasonable for the product they provide. They have held up this long, I’d say they are of good quality. I’ve just ordered another set for a visit to my mothers house! Our favorite pillows!
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Reviewed in the United States on May 23, 2026
★★★★★ 5
Super Comfortable and Easy to Sleep On
Color: Memory Pillow, Size: Standard
HIMOON Shredded Memory Foam Pillows are honestly way more comfortable than I expected—they’re soft but still supportive, and I like that I can adjust and fluff them to fit how I sleep. They feel good for both side and back sleeping, and they don’t go flat like some cheaper pillows I’ve tried. After using them for a while, they’ve held up well and still feel cozy every night. Overall, just a really comfortable, no-stress pillow that makes it easy to fall asleep 👍
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Reviewed in the United States on April 25, 2026
★★★★★ 4
Good pillows; wear a mask when fluffing and airing out
Color: Memory Pillow, Size: Standard
Searched everywhere for comfortable pillows, finally found this set. Perfect density and firmness, very comfortable. Downside is that they were covered in some type of white powder which dispersed as I was fluffing them up. Fiberglass, maybe? I recommend customers fluff and air them outside before use, and wear a mask. Pillows had an industrial smell, which dissipated after 20 minutes in the fresh air.
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Reviewed in the United States on April 8, 2025
★★★★★ 5
Great pillows
Color: Black(4pack), Size: Standard
These are absolutely great and them being cooling pillows is a big plus for someone who has hot flashes at night. They are very comfortable and they are great support for any position sleeper. These pillows are durable and the material comfortable and soft.
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Reviewed in the United States on March 29, 2026
★★★★★ 5
Soft pillows
Color: Grey, Size: Standard
Good size pillows. Very comfortable. Soft pillow and not so hard to lay on. It’s not too bulky or too big.
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Reviewed in the United States on May 15, 2026