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Description
Organotypic balb/c mouse normal small intestinal organoid culture KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information (2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use (3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information (2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use (3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm (4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time) (5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion (6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes (2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4 b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes (2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A. |
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| Synonym | Mouse(Balb/C) Small Intestine Organoid Culture Kit | ||||||||||||||||
| Description | Composition:
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| Storage Temp. | 4 ℃, 3months; -20 ℃, 1 year, see the label for details |
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4.2 ★★★★★
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Product Reviews
★★★★★ 5
Convenient and easy seasoning
Color: Rechargeable Set with Base
I bought these to replace a set of manual twist grinders which didn't last long. Not sure how long these will last but I will say that I am very impressed after my first use. The salt grinder grinds very evenly and it's really easy to see how much is being dispensed. Same with the pepper grinder. I also like that they come with a tray which catches the inevitible particles that would otherwise would scatter on the countertop. Rechargeable, comes with two cords and a cleaning brush and grinders seem to be of high quality. So easy to use - just hold over your steak, burger, fish and press the button with your thumb. Highly recommended.
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Reviewed in the United States on October 17, 2025
★★★★★ 5
Good buy
Color: Rechargeable Set with Base
I bought these for my son and his wife for Christmas this year. He loves them and uses them regularly. Super good looking, easy charging. Nice flow of ground salt and pepper. I have seen him using them while cooking so I asked what he thought.
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Reviewed in the United States on April 26, 2026
★★★★★ 5
Everyone Loves My Electric Salt & Pepper Grinder Set!
Color: Rechargeable Set with Base
Very nice looking grinder set. Rechargeable is the only way to go. They function with ease. I Love that I can easily adjust the size of the grind for both the salt & pepper. It is also nice to have a holder for them to keep counters clean. My family and friends have ask me to send them them the Amazon link so that they can purchase a set for themselves.
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Reviewed in the United States on March 5, 2026
★★★★★ 5
Great Product!
Color: Rechargeable Set with Base
Works as expected. Truly happy with the price and durability so far.
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Reviewed in the United States on March 17, 2026
★★★★★ 4
Good pepper grinder
Color: Rechargeable Set with Base
Good grinding capabilities. When I use for salt ,it drips every where . Only suitable for pepper
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Reviewed in the United States on September 11, 2025