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Description
Rat EERa ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Gently wash adherent cells with pre-chilled PBS, then trypsinize and collect the cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with pre-chilled PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Collect the supernatant for analysis, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an estrogen-related receptor alpha (EERa) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of estrogen-related receptor alpha (EERa) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Estrogen-related receptor alpha ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Estrogen-related receptor α (ERRα), also known as NR3B1 (nuclear receptor subfamily 3, group B, member 1), is a nuclear receptor encoded by the ESRRA (estrogen-related receptor α) gene. It was originally cloned based on DNA sequence homology to estrogen receptor α (ERα, NR3A1). Currently, ERRα is considered an orphan nuclear receptor. It has a wide tissue distribution, but expression is highest in tissues that preferentially use fatty acids as an energy source, such as the kidney, heart, brown adipose tissue, cerebellum, intestine, and skeletal muscle. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids |
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4.5 ★★★★★
Based on 20 reviews
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Product Reviews
★★★★★ 5
Solid; long lasting
I use this adaptor to hard wire an ethernet internet connection straight into a Samsung Galaxy S10+ with PD (charges phone while connected). I also use an S8+ which works, too.
This is a great option for those of us who understand the dangers of being exposed to low-powered microwaves all day and night (AKA Wi-Fi). While hard wired to ethernet, you can enjoy the internet with airplane mode on, which is a big plus!
I've used several different branded adaptors (the ones without PD kill your device's battery QUICKLY) for this purpose, and this Anker outshines them all. Most other brands (I've tried Belkin, Ugreen, and some other somewhat obscure brands) work fine for a short time and then begin to get spotty, losing and regaining connection intermittently until crashing and not working at all over a short period of time, usually not longer than 3 to 4 months if you're real lucky.
However, I've had this Anker for nearly a year (10 months and change), and it's still going strong.
Now, it's not perfect. It does lose connection from time to time and then regain connection just like the others, but nowhere near as frequent, and if you are mindful of the orientation of the cords and take care not to twist or put torque on them once they're connected to a device, it usually keeps connection very nicely.
Be sure to put the device in the proper orientation through which you'll be looking at it, and THEN connect the adaptor (don't connect the adaptor and then have to roll the phone over to look at the screen, as this is the fastest way to kill the adaptor and loosen your phone's charging port).
When first attaching, I plug the ethernet cable into the adaptor and use a hair-tie rubber band to wrap around the end of the ethernet cable, making sure it's tight with no slack, then I secure it around the BASE of the type-C cable coming out of the opposite end of the adaptor (see attached picture). Then LEAVE IT. If you incessantly disconnect and reconnect this adaptor, I guarantee it will not last as long.
As for the features of this adaptor, I can only speak on the ethernet connection, the PD port, and the type-C 5gbps port.
The PD port is nice because if you try connecting to the internet and doing pretty much anything without it, your device's battery is going to drain quite rapidly. How fast depends on how good your device's battery is to begin with. S10+ probably won't last half the time without PD as it does with PD. Just realize that your device is not going to gain power while charging through the PD port, but rather the battery will merely die at a slower pace, giving you more time before having to recharge your device. The other factor is how long your charging cable is (the shorter it is, the longer the battery will last).
Tip: While using PD port, your device will not warn you of your battery about to die since it is technically charging, so if you're doing something important, you had better keep an eye on your battery percentage, lest it dies unexpectedly.
I've not used the hdmi port at all, so nothing to speak on there.
The 5gbps type-c port is nice if you want to connect an external hard drive while either A: connected to the internet, B: still being able to charge your phone, or both! I use the Samsung Portable SSD T7, and the Anker is great for saving large videos/files straight from the internet onto the external hard drive. The Anker is also good for freeing up space on your phone by moving existing files to the hard drive while continuing to charge your phone (otherwise, the hard drive connects directly to your phone, so your charging port is being used, and if your battery is low, no charging). Also, if you disconnected the ethernet cable from the Anker while moving files to an external drive, I suspect that the PD port would actually add battery percentage instead of merely slowing down the loss of battery percentage.
To summarize:
- Don't get Wi-fried: use a hard-wired ethernet connection instead!
- Great adaptor that lasts longer than all others I've tried.
- PD is a must have feature, but your device will still eventually die.
- Great for saving videos/large files straight from internet to external hard drive while being able to charge device simultaneously.
This Anker is the one you're looking for!
Cheers.
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Reviewed in the United States on December 15, 2022
★★★★★ 5
Excellent quality with just the right amount and type of connection points I needed!
Extremely good quality. I've used many Anker product over the years and this one didn't disappoint! Needed it to run a laptop on my network in isolation mode and to have accessible connections for ethernet as well as a wireless mouse and extra cooling fan as my laptop has only 1 standard size USB 3.0 port. This is the mini version so It won't necessarily supply enough juice to charge your laptop at the same time (there's another 8 in 1 made by Anker for that) but it can supply enough power to maybe get you through a very trying session, very short term. Super-easy to connect and just works. Fits comfortably in my carry bag too. Very light weight, good heat dissipation although I didn't really have any or at least not much. Compatible with all the connectors you see in the picture. In my case at least, it wasn't a luxury it was a necessity and it proved it's value immediately.
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Reviewed in the United States on April 26, 2026
★★★★★ 5
USB ports charge.
Relatively cheap, lightweight, and better than most. It gets warm, but not hot. The Video adapter was immediately recognized by my Macbook and works flawlessly at all resolutions made available (much higher than Windows PCs) I used it both as the recipient of my 65W Mac charger, and the Mac received its power from this device; I also had the 65W Mac charger plugged directly into the Mac, and this plugged into the second USB-C plug on the mac. It works (HDMI monitor) both ways. It says that the ports are not for "charging", however be aware that they do charge when devices are connected, for example my iPhone to the extra USB-C port, or the USB-A port. My Logi back-lighted keyboard lights up too. I don't know why they say it is not for charging. Perhaps it is simply saying there are no ports expressly intended to do charging without connecting to your computer.
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Reviewed in the United States on February 18, 2026
★★★★★ 5
High quality and all the right connection types
This works great. I've used it for both my MAC and PC. I really love the Ethernet on the end and the HDMI connections (which modern laptops don't include at times). This is a great adaptor and highly recommend.
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Reviewed in the United States on May 14, 2026
★★★★★ 4
One complaint
Works very well and solved port issues on my X1 Carbon. My only quibble is that the C interface cable from the unit to the PC is a bit too stiff and tends to lose connection if things move about.
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Reviewed in the United States on December 5, 2025