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Description
Mouse Intestinal Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) put the normal intestinal tissue of mice after sampling into the sampling bottle of pre cooled (2 8 c) tissue preservation solution e, and quickly transfer it to the clean laboratory for tissue processing and stem cell separation, take photos and register information (2) prepare several Petri dishes and add 4 pre cooled primary culture buffer B for standby (3) disinfect the sampling bottle, put the tissue
Product Specification
| Usage |
1. Primary (1) put the normal intestinal tissue of mice after sampling into the sampling bottle of pre cooled (2-8° c) tissue preservation solution e, and quickly transfer it to the clean laboratory for tissue processing and stem cell separation, take photos and register information (2) prepare several Petri dishes and add 4 ℃ pre cooled primary culture buffer B for standby (3) disinfect the sampling bottle, put the tissue into the Petri dish, wash it three times with primary culture buffer B, remove impurities, remove the intestinal mucosa of small intestine tissue with ophthalmic scissors or scalpel, and cut it into a tissue block with a volume of about 1-3mm3 (4) the tissue was digested with the primary tissue digestive solution c of mouse normal intestine, and then it was shaken at 4 ℃ for 10min (the digestion condition was observed at any time during the digestion process) (5) take a small amount of liquid and observe it under the microscope. After observing more single cells or cell clusters below 70UM under the microscope, add three times the volume of primary culture buffer B to stop digestion (6) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation (7) Matrigel calculation: after step 6, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate (8) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃) (9) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a glue, and add mouse normal intestinal organoid medium a (restore room temperature) for culture 2. Organoid subculture (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4  &Nbsp&Nbsp&Nbsp b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4 (4) after organ collection, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul of mouse normal intestinal organoid culture medium a 3. Organoid cryopreservation (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid (4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube (5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage 4. Organoid resuscitation (1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube (2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting (3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min (4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g (5) resuspend Matrigel, lay 25ul Matrigel per well in a 24 well cell culture plate, place in an incubator for 10-15min to form a gel, and add 500ul of mouse normal intestinal organoid culture medium A. |
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| Description |
Composition:
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| Storage Temp. | 4 ℃, 3months-20 ℃, 1 year, see the label for details |
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4.0 ★★★★★
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Product Reviews
★★★★★ 5
Perfect Size - No Squeakers (yay)
Color: Pink, Size: MINI 1.75" - 4 Pack
My 9 lb. Chi-Mix adores her little toy balls. Perfect for her small size (or a medium dog) and full of bounce. I didn't want squeakers because I knew it would drive me nuts. No squeakers in these balls. Perfect training tool to teach fetch. Perfect toys to have play time with your pooch.
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Reviewed in the United States on April 14, 2026
★★★★★ 4
Nice and bright
Color: Orange, Size: MINI 1.75" - 4 Pack
These are great to fit the ball shooter I use with my dogs. Color orange makes it easy to find in grass or dirt. Problem is I have a chewer. He loves to chew on the ball and eat the orange fiber. So, the balls get wrecked and I have to replace. Gets costly. I wish they made this same size ball without the orange furry tennis-ball like covering. Maybe the toys would last longer.
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Reviewed in the United States on April 21, 2026
★★★★★ 5
Safe for dogs
Color: Green, Size: Regular 2.5" - 4 Pack
Much better for dogs than used/dirty tennis balls. Worth the money for safety.
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Reviewed in the United States on May 27, 2026
★★★★★ 3
Easily breakable coating/fur
Color: Pink, Size: MINI 1.75" - 4 Pack
I wanted to love this item but sadly my dog really messed up the coating/fur on these balls so easily, other than that great product but with her being a puppy I got these smaller ones for that reason and I’m absolutely scared of her destroying things and getting stomach issues, so it’s just not worth it to me.
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Reviewed in the United States on April 20, 2026
★★★★★ 5
Perfect size
Color: Orange, Size: MINI 1.75" - 4 Pack
These were the perfect size for my ball chasing kittens. they love scuffing up the balls and carrying them around the house or bringing them to me to play fetch. Yes, my cats love to play fetch.
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Reviewed in the United States on May 12, 2026