Rat IL-4 ELISA Kit

Testing Principle

This kit uses quantitative sandwich enzyme immunoassay technique. A specific anti-rat IL-4 antibody is pre-coated on a high affinity plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme-labeled plate. After incubation, the IL-4 present in the samples combines with the solid-phase antibodies and the detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin (HRP) was added. After washing, a chromogenic substrate was added, and the color was developed in the dark. The reaction was stopped by adding a stop solution, and the absorbance was measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm).

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

96 well polysyrene microplate coated with capture antibody, standard, detection antibody, 10×reagent diluent, Color reagent (A and B), 25×wash buffer, Stop solution, ELISA plate sealers, specification

Sensitivity:  2.3 pg/mL

Detection range:   15.6 - 1,000 pg/mL

Recovery rate:  75-112%

Storage: 2-8 ° C

The standard curve:

Background: IL-4

Il-4 is a secretory protein of 129 amino acids.Il-4 is a cytokine produced mainly by activated T lymphocytes, mast cells and basophils.By acting on a variety of cell types, IL-4 regulates a variety of immune responses. Il-4 is involved in several B cell activation processes and induces class II MHC molecules to be expressed in dormant B cells.It is also a common irritant in DNA synthesis. Il-4 can enhance the secretion and cell surface expression of IgE and IgG1.It also regulates the expression of low affinity Fc receptors for IgE (CD23) on lymphocytes and monocytes.Il-4 positively regulates the expression of IL31RA in macrophages.

Technical hints: For research use only, not for in vitro diagnosis.