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Description
UniOne® TR-FRET Human BCL6/CRBN PROTAC Binding KitProduct Specification Host Human Stability & Storage Store the kit at 80C or below. When storedappropriately, the reagents remain stable until the expiration date marked onthe label. Once thawed, BCL6 and CRBN stock solutions can undergo oneadditional freeze thaw cycle; labeled antibody solutions can undergo oneadditional freeze thaw cycle. To avoid repeated freeze thaw cycles, it isrecommended to aliquot the remaining stock solutions into single use
Product Specification
| Host | Human |
| Stability & Storage |
Store the kit at -80°C or below. When storedappropriately, the reagents remain stable until the expiration date marked onthe label. Once thawed, BCL6 and CRBN stock solutions can undergo oneadditional freeze-thaw cycle; labeled antibody solutions can undergo oneadditional freeze-thaw cycle. To avoid repeated freeze-thaw cycles, it isrecommended to aliquot the remaining stock solutions into single-use containersfor storage at -80°C. Thawed diluents and Detection Buffer can be stored at2-8°C. |
Background
This kit uses Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the molecular glue ARV-393 and testcompound-mediated interaction between the DDB1/CRBN complex and BCL6. Thismethod enables simple and rapid high-throughput screening of small moleculescapable of mediating the interaction between the DDB1/CRBN complex and BCL6.
As shown, the interaction between DDB1/CRBN and BCL6 isdetected using a Eu-labeled Anti-Tag1 Antibody (TR-FRET donor) and anAcceptor-labeled Anti-Tag2 Antibody (TR-FRET acceptor). As the molecular glueARV-393 mediates the interaction between DDB1/CRBN and BCL6, the donor andacceptor antibodies are brought into proximity. Excitation of the donorantibody triggers Fluorescence Resonance Energy Transfer (FRET) to the acceptorantibody, resulting in a specific emission signal at 665 nm. This specificsignal is proportional to the degree of interaction mediated by ARV-393 betweenDDB1/CRBN and BCL6. This homogeneous assay is simple to perform and requires nowashing steps.
Components
Table 1. Kit Components and Storage Conditions
Component |
Concentration |
100 TESTS |
500 TESTS |
2500 TESTS |
10000 TESTS |
Storage Temperature |
|---|---|---|---|---|---|---|
Tag1-DDB1/CRBN protein, |
50× |
10μL |
50μL |
250μL |
1000μL |
-80℃ |
Tag2- BCL6 protein |
50× |
10μL |
50μL |
250μL |
1000μL |
-80℃ |
ARV-393 (100 μM) |
100× |
5μL |
25μL |
125μL |
500μL |
-80℃ |
Anti-Tag1 Eu dye antibody |
50× |
10μL |
50μL |
250μL |
1000μL |
-80℃ |
Anti-Tag2 Ac antibody |
20× |
25μL |
125μL |
625μL |
2500μL |
-80℃ |
Detection buffer |
1× |
2ml |
10ml |
10mL |
40mL |
-80℃ |
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