Mouse IL-2 High Sensitivity ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti- mouse IL-2 capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-2 in the sample binds to the solid-phase antibody. After thorough washing to remove free and unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-2 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, biotin labeled IL-2 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.05 pg/ml

Detection Range: 0.23-15 pg/ml

Recovery Range: 82-98%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 2 (IL-2) is a 15kDa glycoprotein. In humans, it is encoded by a single gene located in the q26– 28 region of chromosome 4. It is a class of signaling molecules in the immune system, which regulates the immune response of leukocytes (leukocytes, usually lymphocytes) with immune functionActivity of. IL-2 is the body's natural response to microbial infection, distinguishing foreign and self antigens. IL-2 functions by interacting with the IL-2 receptor, which is composed of three chains, namely α Chain (CD25), β Chain (CD122) and γ Chain (CD132).