SKU: 79574456571

Human Pancreatic Organoid Culture Medium Kit

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Description

Human Pancreatic Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it

Product Specification

Usage 1. Primary
(1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm<(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)<(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion<(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge
(7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board
2. Organ like passage culture
(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)
(3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4
&nbsp& Nbsp& Nbsp& Nbsp& Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4
3. Organ like cryopreservation
(1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group)
(3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid
(4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube
(5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage
4. Organ like resuscitation
(1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A.
Description Composition:
Name Specification
Human normal pancreatic organoid medium A 100ml
Primary culture buffer B 250ml
Human normal pancreas primary tissue digestive juice C 30ml
Organoid passage digestive juice D 30ml
Tissue preservation solution E 100ml
Organoid cryopreservation fluid F 20ml
Organoid subculture buffer G 250ml
Storage Temp. 4° C. 3months; -20° C. 1 year, see label for details
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SKU: 79574456571

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EchoNaturx
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★★★★★ 5
Finally a razor for women that's gentle where it matters and powerful where it counts.
I could tell right away that this razor was designed with women in mind. It petite enough to fit in curves, but not so small that you spend all day grooming. Bonus feature - unlike some razors you can charge it with or without the stand. For someone on the go, having a rechargeable razor that's compact for travel and uses the same cord as my phone is a HUGE win. Other things I liked: it has a nice touch to it and it balances well in my hand. It's easy to hold and maneuver. You can use it both as a trimmer for quick shaping and for a close shave. A note on this: Any shaver will pull hairs if you use it incorrectly. Start with the straight trimmer for longer hairs. Watch your angle and adjust as needed. Do an internet search for videos to help. Use the guards if your hair is especially long. Only switch to the round shaver with really short hair or stubble. I had zero issues with pulled hairs. It truly is painless as advertised. The shave quality was close and baby smooth with zero skin irritation. It functions exactly as advertised and the battery life was excellent. Truth be told, it's winter so it's been a while since I last shaved my legs. I needed the extended time. Having both a trimmer and razor in one tool is fantastic value and time saver. Clean up was simple. I'm happy to finally have a women's razor that works as it should.
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Reviewed in the United States on January 31, 2026
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Amazon Customer
Cuba, US
★★★★★ 5
Gentle, Waterproof & Super Convenient 2-in-1 Trimmer
I’m really happy with this VOGCREST Bikini Trimmer & Facial Hair Shaver. The 2-in-1 design is very practical—one head works great for bikini area trimming, and the facial shaver is perfect for quick touch-ups. It’s gentle on the skin and truly lives up to the “no irritation” claim. The device feels well-made, runs smoothly, and is easy to use. I especially like that it’s IPX7 waterproof, so cleaning it is simple and stress-free. The safety design gives confidence when trimming sensitive areas, and the results are neat without pulling or discomfort. Overall, this is a great value for the price. Compact, hygienic, and effective—definitely recommended for anyone looking for an all-in-one grooming tool. ⭐⭐⭐⭐⭐
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Reviewed in the United States on January 9, 2026
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Nik
Belleville, US
★★★★★ 5
Works really well. Great value for less
I'm genuinely impressed with the quality and performance it offers. From the moment I unboxed it, the product felt substantial and well-made, a clear indicator of the high-quality materials used. This is a device that feels like it's built to last. The performance lives up to the initial impression. The battery life is excellent, easily handling multiple uses without needing a recharge. When it does need power, the dual-charging option—either directly with a cable or through the convenient charging station—is a fantastic feature that adds to its versatility and ease of use. The assortment of included shaver heads and attachments is particularly impressive, offering a wide array of options that will likely satisfy any grooming need.
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Reviewed in the United States on January 15, 2026
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Priscila R.
Omaha, US
★★★★★ 5
Great Design, Long Battery Life, and Excellent Performance
I really like this product. It’s nice-looking, well-finished, and pleasant to the touch. It’s very versatile! you can charge it on its stand or plug it directly into the wall if you want to bring it on a trip and skip the stand. I’ve used it several times and only had to recharge it once. the battery lasts a long time. It trims gently without irritation, works well on both body and facial hair, and the waterproof design makes it easy to clean. It’s lightweight, easy to handle, and great for quick touch-ups without any hassle. Overall, a very good value for the price.
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Reviewed in the United States on December 5, 2025
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Nayelle Carvalho Jales
Lowell, US
★★★★★ 5
Excellent quality and looks beautiful
I really loved this product! I’ve already used it twice and it works excellently. The design is beautiful and feels very high-quality. The battery lasts a long time, which is a big plus, and the power is just right. One of the things I liked most is that it doesn’t hurt at all. I’ve tried other trimmers before that irritated my skin, and this one is very gentle. The size is perfect, compact and easy to pack, making it great for travel. Even my husband tried it and really liked it, which shows how versatile and well made this device is. Overall, I’m very happy with this purchase and would definitely recommend it.
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