Pay in installments of $35.75 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 28 - Sep 2
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Annexin V-EGFP/PI Apoptosis analysis kitProduct Specification Usage 1. Adjust the cell density to about 1 106Cells ml. 2. Remove 0. 2 ml of cell suspension from a cell culture flask (2 105Cells) were transferred into a clean centrifuge tube. 3. Centrifuge at 1000 g at room temperature for 5 minutes to remove the medium. 4. The cell pellet was gently resuspended with 0. 2 ml of pre cooled PBS solution. 5. Centrifuge at 1000 g for 5 minutes at room temperature to remove PBS. 6. The cell
Product Specification
| Usage | 1. Adjust the cell density to about 1 × 106Cells/ml. 2. Remove 0.2 ml of cell suspension from a cell culture flask (2 × 105Cells) were transferred into a clean centrifuge tube. 3. Centrifuge at 1000 g at room temperature for 5 minutes to remove the medium. 4. The cell pellet was gently resuspended with 0.2 ml of pre-cooled PBS solution. 5. Centrifuge at 1000 g for 5 minutes at room temperature to remove PBS. 6. The cell pellet was gently resuspended with 0.2 ml of pre-cooled binding buffer (component C. Binding Buffer). 7. Add 5μl Annexin V-EGFP (component A) and 5μl PI dye solution (component B), and gently mix well. 8. Incubate at room temperature (18-24 °C) for 10-15 minutes in the dark. 9. Alternatively, replenish the volume to 0.4 ml with binding buffer or PBS. 10. Place the sample on ice and protect it from light, and test it as soon as possible. 11. Use flow cytometry or fluorescence microscope for detection and analysis. | ||||||||||||||||
| Beilstein | 0 | ||||||||||||||||
| Synonym | Annexin V-EGFP/PI Apoptosis Analysis Kit | ||||||||||||||||
| Description |
Annexin V-EGFP/PI cell apoptosis detection kit uses EGFP-labeled Annexin V as a probe to detect the occurrence of early cell apoptosis. The detection principle is: in normal living cells, phosphotidylserine (PS) is located on the inside of the cell membrane, but in early apoptotic cells, PS flips from the inside of the cell membrane to the surface of the cell membrane and is exposed to the extracellular environment. Annexin-V (Annexin-V) is a Ca2 +-dependent phospholipid binding protein with a molecular weight of 35-36KD, which can bind to PS with high affinity. It can bind to the cell membrane of early apoptotic cells through phosphatidylserine exposed on the outside of the cell. In addition, Propidium Iodide (PI) is also provided in this kit to distinguish surviving early cells from necrotic or late apoptotic cells. PI is a nucleic acid dye that cannot penetrate the intact cell membrane of normal cells or early apoptotic cells, but can penetrate the cell membrane of late apoptotic and necrotic cells to make the nucleus red. Therefore, when Annexin V is used in combination with PI, PI is excluded from viable cells (Annexin V-/PI-) and early apoptotic cells (Annexin V +/PI-), while late apoptotic cells and necrotic cells are stained double positive by EGFP and PI combination (Annexin V +/PI +).
Product Components:
|
||||||||||||||||
| PubChem CID | 0 | ||||||||||||||||
| Storage Temp. | Stored at 2-8 ℃ in the dark from light, the validity period is 6 months. Store at-20 ℃ in the dark from light, shelf life for 12 months or more, avoid repeated freezing and thawing. |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.4 ★★★★★
Based on 20 reviews
Sort
Product Reviews
★★★★★ 5
great water shoe!
Size: 7 Women/6 Men, Color: 3f-410 Rose Red
I absolutely loved these water shoes! They are surprisingly very comfortable and slip resistant. The sole is very thick and the material is pretty quick to dry. I purchased them for my cruise port excursions and wore them dry, in the water, and walked around after being in the water—no blisters or uncomfortable rubbing. I would say they are maybe a little on the larger side for women. I can range anywhere from a 7, 7.5 to an 8. I purchased a size women’s 7/men’s 6 and it was perfect. I am excited to wear these at the beach and in the creek this summer!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 5, 2026
★★★★★ 5
Great Find
Size: 8, Color: Blue
Cute, comfortable, colorful have soft straps. I was very surprised by the both the quality and comfort of these Flip Flops. The price is so affordable that I bought several pairs in other colors. They are light weight, have ample arch support, fit true to size and can be worn with jeans, short or a casual dress. They really do look just as good as any of the high-priced brands.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 20, 2026
★★★★★ 5
Overall, these are some of the most comfortable flip flops I have owned, and would buy again.
Size: 6.5, Color: White Khaki
I was so happy with these KuaiLu Flip Flops that I bought two more pair, one in black and one in brown! They were comfortable right out of the box and did not need any break-in time. The arch support is excellent and gives my feet much better support than regular flip flops. They are also light weight, well made, and cute enough to wear with many different outfits. The soles have good traction, and they feel sturdy when walking.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 28, 2026
★★★★★ 5
Nice Flip Flops for the Price!
Size: 7.5, Color: White Khaki, Size: 7.5, Color: White Khaki
I just received my flip flops and tried them on, and they have a nice cushiony feel to them. The white color matches many of my outfits great. And while the fit is generally good, they could be a bit wider as I have wide feet. But they still feel comfortable.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 18, 2026
★★★★★ 4
runs big
Size: 7.5, Color: Camel
I am always a size 8 in boots, sandals, sneakers and read these ran large so ordered a size 7.5. They are still too big on me. I sent back for a 7 and I will update when I get those. They are very, very cute and comfortable! I love the cloth that goes over the foot!! That makes them seem a bit more fancy than regular flip flops. They also do sound like regular flip flops, so if you want a quiet sandal these are not for you. They are very cushioned and I can def feel the arch support. You will need to size down at least half size and maybe one whole size. The hardest thing for me was to pick a color! May have to get several pair! Just ordered my mom the dark blue and I know she will love them!!! Highly recommend, especially for the low price!
Update: Got the 7 and are still too big. As I said, I got my mom the 6 so I tried them on and they are still a tad too big on me!!! BTW the sizes 8,7.5,7 and the 6 I can hardly see any difference whatsoever! They don't come in 5 1/2 so I am sending the 7 back for a 6 and keeping those just because they are so darn cute and comfortable! I also don't have a very thick foot so I wish the straps were a tad tighter, but I will deal with it. So, to sum up: Be sure to size down at least one size and maybe even two. Returns are easy, so maybe try a few different sizes! I haven't worn a size 6 since I was in about the 6th grade, so not sure what is going on with the sizing. Oh yeah, I did get three different colors and the sizing was the same with all three. HTH! Good luck!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 26, 2026
recommand products
PROACT Metal Max Forceps, Magill, Disposable, 170mm (Paediatric) PACK OF 10
26.99
Classic Height Adjustable Armrests For Operators Chair (1004)
51.99
Polar Thermal Wheeled Trolley for use with vaccine bags
62.99
Showa Biodegradable Nitrile Disposable Gloves - Extra Large - Pack of 100
12.96
MINI-PLASCO LIDOCAINE 2% 10ML (PLASTIC AMPOULE) - X 20
24.15