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Description
Bak Recombinant Rabbit mAb (S-2990-55)Product Specification Host Rabbit Antigen Bak Synonyms Bcl 2 homologous antagonist killer; Apoptosis regulator BAK; Bcl 2 like protein 7 (Bcl2 L 7); BAK; BCL2L7; CDN1; BAK1 Immunogen Synthetic Peptide Location Mitochondrion Accession Q16611 Clone Number S 2990 55 Antibody Type Recombinant mAb Isotype IgG Application WB Reactivity Hu, Ms, Rt, Mk Positive Sample HeLa, THP 1, Jurkat, HT 29, Molt 4, RPMI 8226, NIH 3T3, A20, rat spleen, COS 7 Purification
Product Specification
| Host | Rabbit |
| Antigen | Bak |
| Synonyms | Bcl-2 homologous antagonist/killer; Apoptosis regulator BAK; Bcl-2-like protein 7 (Bcl2-L-7); BAK; BCL2L7; CDN1; BAK1 |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | Q16611 |
| Clone Number | S-2990-55 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, THP-1, Jurkat, HT-29, Molt-4, RPMI-8226, NIH/3T3, A20, rat spleen, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
Bak is a pro-apoptotic member of the Bcl-2 protein family that localizes to the mitochondrial outer membrane, where it functions as a key executor of apoptosis; upon activation by BH3-only proteins or other stimuli, Bak undergoes conformational changes, oligomerizes, and forms pores in the membrane, leading to the release of cytochrome c and other apoptogenic factors into the cytosol to trigger programmed cell death.
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