SKU: 82252179712

Human IL-12p70 High Sensitivity ELISA Kit

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Description

Human IL-12p70 High Sensitivity ELISA KitProduct Specification Species Reactivity Human Synonym CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL 12 p70, IL 12 subunit p35, IL 12, subunit p35, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin 12 alpha chain, interleukin 12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain

Product Specification

Species Reactivity Human
Synonym CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL-12 p70, IL-12 subunit p35, IL-12, subunit p35, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, p35
Description

Detection principle:

This kit uses double antibody sandwich ELISA Technologies. Specificity anti- Human IL-12 capture antibody Pre-coated on high affinity enzyme plates. The standard substance, the sample to be measured and the biotin-labeled detection antibody are sequentially added into the wells of the enzyme label plate, thoroughly shaken and mixed, and then placed at room temperature for time 2 Hours of incubation process, present in the sample IL-12 Binds to solid phase antibodies and detection antibodies. After sufficient washing to remove free and unbound components, horseradish peroxidase-labeled streptavidin was added (Streptavidin-HRP , SA-HRP) 。 After washing, the signal enhancer was added for incubation, and after washing to remove unbound material, it was added again SA-HRP 。 After washing again, add TMB The chromogenic substrate is incubated at room temperature in the dark from light to develop color. The depth of color reaction and the sample IL-12 The concentration of is positively correlated. Add a stop solution to stop the reaction, and use a microplate reader to 450 nm Detection wavelength ( Correction wavelength 570-630 nm) The absorbance values were determined under conditions.

 

Test Type: Double antibody sandwich method

 

Form: Pre-coating 96 Orifice plate

 

Test Sample Type: Cell supernatant, serum, plasma

 

Sample Load: 100 μl

 

Kit Components: Pre-coating 96 Well plates, standards, IL-12p70 Test antibody, standard dilution, test buffer, signal enhancer, signal enhancer dilution, TMB A chromogenic substrate, a wash liquid, a stop liquid, SA-HRP , a copy of the sealing film and instructions.

 

Sensitivity: 0.04 pg/mL

 

Detection range: 0.39-25 pg/mL

 

Recovery Range: 91-104%

 

Save Method: 2-8℃

 

Standard graph:

 

 

Background:

Interleukin 12(IL-12) Composed of dendritic cells, macrophages, neutrophils and human B Lymphoblastic cell line (NC-37) Arises naturally in response to antigenic stimulation. IL-12 By 4 1 а Helix composed, is a composed of 2 Species independent gene ---IL-12A(p35) And IL-12B(p40) Encoding the formed heterodimeric cytokine. Biologically active heterodimers can be formed after protein synthesis (p70) And homodimers p40 。 IL-12 Participate in initial T Cells to Th1 Process of cell differentiation, as T Cell stimulating factor, which stimulates T The growth of cells and their functions. IL-12 Has anti-angiogenic activity, and is effective against natural killer cells and T Lymphocyte activity plays an important role. Similarly, IL-12 Associated with autoimmunity.

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SKU: 82252179712

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AGraber89
Cuba, US
★★★★★ 5
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Format: Board book
Both of my daughters have loved this book. I bought it initially for my 12 year old (as a toddler) and she adored the different animals voices I would do. Bought it again for my 18 month old and she just giggles and giggles.
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Reviewed in the United States on March 29, 2025
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Heather
Phoenix, US
★★★★★ 5
Love Mr Panda
Format: Board book
Love this book. My nanny kiddos now remember to say please, though they took it kinda literally and if you don't say please they will refuse you in return, which is good they learn to set their own boundaries! Fun, easy to read, flows, and gives the opportunity to talk about setting boundaries and being polite!
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Reviewed in the United States on November 23, 2023
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Esther Maguire
Massapequa, US
★★★★★ 5
Greatest Book Ever
I have ordered this book over 12 times for baby showers. I love this illustrations and every principal it stands for. Ignore the haters. I think it’s wonderful to instill in children a core foundation of civility in a society that has a proclivity of demanding what they want. I know a few adults who could learn something from this book today.
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Reviewed in the United States on March 29, 2022
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Ashley S
Cuba, US
★★★★★ 5
Our son LOVES this book
Format: Board book
Our son fell IN LOVE with this book from the first read. He is now always saying PLEASE Mr. panda. He asks us to read it multiple times a day.
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Reviewed in the United States on January 26, 2025
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Caroline
New York, US
★★★★★ 4
Book is a hit but not my favorite
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My child likes this book and asks for me to read it to her. I find myself changing some words like you "may" have a donut instead of you "can" have a donut, and making the animals asking for donuts ruder or changing my tone of voice. Nice illustrations, and again -- it's a book that she asks to be read.
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Reviewed in the United States on August 19, 2024

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