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Description
Mouse IL-10 OneStep ELISA KitProduct Specification Antigen IL 10 Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Ms Purification Protein A Stability & Storage 2 to 8 C as supplied. Kit Precision Intra assay: 5. 8% Inter assay: 8. 6% Sample type Cell culture supernatant, Serum, Plasma Assay type Sandwich (quantitative) Sensitivity 0. 057 ng ml Range 0. 16 ng mL 10 ng mL Recovery Cell culture supernatant: 87% Serum: 95% Plasma: 95% Assay time 60 minutes
Product Specification
| Antigen | IL-10 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Precision | Intra-assay: 5.8%； Inter-assay: 8.6% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.057 ng/ml |
| Range | 0.16 ng/mL – 10 ng/mL |
| Recovery | Cell culture supernatant: 87% Serum: 95% Plasma: 95% |
| Assay time | 60 minutes |
| Species reactivity | Ms |
Background
Interleukin 10(IL10), also known as cytokine synthesis inhibitory factor (CSIF), is a secreted protein and belongs to the IL-10 family. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. IL-10 is an anti-inflammatory TH2 cytokine that has a critical role in limiting the immune response to pathogens to prevent host damage. As IL-10 in produced in several T helper populations, it is proposed that it provides a feedback loop to limit the effector functions of macrophages and DCs on T cells. Once expressed, IL-10 signals through the IL-10 receptor (IL-10R) to activate STAT3. As IL-10 is a strong inhibitor of inflammation, it has become a viable biomarker for various diseases and conditions as well as a therapeutic molecule for certain conditions. In addition to elevated levels in parasitic infection, high expression levels of IL-10 are also found in retroviral infections inducing immunodeficiency. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations.
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