SKU: 86620593282

UniOne® TR-FRET Human BRD4/CRBN PROTAC Binding Kit

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Description

UniOne® TR-FRET Human BRD4/CRBN PROTAC Binding KitProduct Specification Host Human Stability & Storage 80 Background This kit utilizes Homogeneous Time Resolved Fluorescence(TR FRET) technology to measure the Molecular Glue BET Degrader 1 and testcompound mediated interaction between the DDB1 CRBN complex and BRD4. BRD4contains bromodomain 1 and 2. This method enables a simple and rapidhigh throughput format for detecting small molecules capable of mediating theinteraction between the DDB1 CRBN

Product Specification


Host Human
Stability & Storage

-80℃

Background

This kit utilizes Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the Molecular Glue BET Degrader-1 and testcompound-mediated interaction between the DDB1/CRBN complex and BRD4. BRD4contains bromodomain 1 and 2. This method enables a simple and rapidhigh-throughput format for detecting small molecules capable of mediating theinteraction between the DDB1/CRBN complex and BRD4.

As shown, the interaction between DDB1/CRBN and BRD4 isdetected using a Eu-labeled Anti-Tag1 Antibody (TR-FRET donor) and anAcceptor-labeled Anti-Tag2 Antibody (TR-FRET acceptor). As the Molecular GlueBET Degrader-1 mediates the interaction between DDB1/CRBN and BRD4, the donorand acceptor antibodies are brought into proximity. Excitation of the donor antibodytriggers Fluorescence Resonance Energy Transfer (FRET) to the acceptorantibody, resulting in a specific emission signal at 665nm. This specificsignal is proportional to the degree of interaction mediated by BET Degrader-1between DDB1/CRBN and BRD4. This homogeneous assay is simple to perform andrequires no washing steps.



Components

Component

Concentration

500 TESTS

2500 TESTS

10000 TESTS

Storage Temp.

Tag1-DDB1/CRBN protein

25×

80μL

400μL

1600μL

-80°C

Tag2-BRD4 protein

100×

20μL

100μL

400μL

-80°C

BET Degrader-1 (100μM)

10μM

10μL

50μL

200μL

-80°C

Anti-Tag1 Eu dye antibody

50×

50μL

250μL

1000μL

-80°C

Anti-Tag2 Ac antibody

12.5×

200μL

1000μL

4000μL

-80°C

Detection buffer

10×

2mL

10mL

40mL

-80°C

*Repeated freeze-thaw cycles should be avoided. Aliquot upon first thaw and store according to the specified conditions. Valid for one year.

Shipping Notes
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Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 86620593282

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4.8 ★★★★★
Based on 19 reviews
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Product Reviews
I
Verified Purchase
InspirationalReader
Natrona Heights, US
★★★★★ 5
Great dog toy! Good quality!
Color: brown1
Great quality dog toy! The rope is thick and not easy to break. Fun toy for my medium-sized dog who loves to tug-of-war with me.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 30, 2025
E
Verified Purchase
Edilla
Boise, US
★★★★★ 3
For small dog
Color: brown4, Color: brown4
It's cute for the price, but it's meant for small dogs, it's very easy for medium-sized dogs to destroy.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 10, 2026
R
Verified Purchase
Robert A.
Belleville, US
★★★★★ 5
Small Pug Enjoyment For All!
Color: brown1, Color: brown1
We got this toy to replace an earlier purchase for a rope/squeak toy after finding he loved a holiday purchase by an Aunt which squeaked but was not really meant for tugging/heavy playing like he wanted too, for our half pug/schnauzer Mr. Pug. Compared to the first toy and the attempted replacement he ADORES this toy! As you can see in the above picture he has truly laid his claim to it! About the product in general though, we found it surprisingly sturdy all around, even with heavy tugging and aggressive play. The interior padding is durable it seems, and holes where the rope come out have a heavy duty padding for the target dog audience like ours in preventing him from chewing or tearing it up. The crinkly ears were also an enjoyable surprise for him, and the squeaker can move around the torso area of the toy keeping him actively looking for it when he desires it. All in all we would certainly get another toy like this in the future.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 25, 2025
T
Verified Purchase
the banquets are super nice and strong
Louisville, US
★★★★★ 5
Perro
Color: brown1
A mi perro le encanto ese juguete, lo recomiendo
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Reviewed in the United States on March 30, 2026
D
Verified Purchase
doughbowlmaker
Omaha, US
★★★★★ 5
Great wrestle or chewing toy for your pet.
Color: brown1
My 4 legged "little boy" loves to play, even at 10 years old but is getting a tad "over the hill" for chasing a ball. This toy has brought him (and me) many hours of fun as it can be played with in the house without chance breaking something (it is soft) and was fairly well thought out - being made with the "squeeker" in one end, and a couple of ropes sewn in to provide a "tug of war" when he is really feeling frisky. I've had it around 6 months and it has only incurred minor damage and really just normal wear and tear considering what it has gone thru. A great toy for the price that your pet may play with by itself, or one you can use to wrestle with it so you can be part of the action. When this one is finally torn apart, I will be buying another one (if the dog out lasts it), or I will bury my pet with this one if it out lasts him. It's that special.
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Reviewed in the United States on January 30, 2026

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