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Description
Pericentrin Recombinant Rabbit mAb (S-3515-69)Product Specification Host Rabbit Antigen Pericentrin Synonyms Kendrin; Pericentrin B; KIAA0402; PCNT2; PCNT Immunogen Recombinant Protein Location Cytoplasm, Cytoskeleton, Centrosome Accession O95613 Clone Number S 3515 69 Antibody Type Recombinant mAb Isotype IgG Application WB, ICC Reactivity Hu, Ms, Rt, Mk Positive Sample HEK293, HepG2, HeLa, MCF7, NIH 3T3, mouse testis, C6, rat testis, COS 7 Purification Protein A Concentration 2 mg ml
Product Specification
| Host | Rabbit |
| Antigen | Pericentrin |
| Synonyms | Kendrin; Pericentrin-B; KIAA0402; PCNT2; PCNT |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Cytoskeleton, Centrosome |
| Accession | O95613 |
| Clone Number | S-3515-69 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HEK293, HepG2, HeLa, MCF7, NIH/3T3, mouse testis, C6, rat testis, COS-7 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, MK |
| ICC | 1:1000-1:2000 | Hu |
Background
Pericentrin (PCNT, also called kendrin or pericentrin-B) is a large, highly conserved coiled-coil scaffold protein encoded by the PCNT gene on chromosome 21, which localizes to the centrosome and pericentriolar material (PCM) via its C-terminal PACT domain, anchoring γ-tubulin complexes and other regulatory proteins to organize microtubule nucleation and anchoring, thereby orchestrating centrosome assembly, mitotic spindle formation, cell-cycle progression and primary cilium biogenesis; mutations or dysregulation of PCNT underlie disorders such as microcephalic osteodysplastic primordial dwarfism type II, Seckel syndrome, ciliopathies, and have been linked to cancer, mental disorders and altered insulin secretion in β-cells.
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