SKU: 11977133429

Human Galectin-3 ELISA Kit

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Description

Human Galectin-3 ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. The specific anti human galectin 3 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. Galectin 3 in the

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. The specific anti-human galectin-3 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. Galectin-3 in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of galectin-3 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, galectin-3 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 2.46pg/ml

Detection Range: 62.5-4000 pg/ml

Recovery Range: 85-105%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Galectin-3It belongs to the lectin family and is expressed in the nucleus, cytoplasm, mitochondria, cell surface and extracellular. Galectin-3 pair &beta-Galactosidase has affinity and exhibits its activity against bacteria and fungi. It is involved in many biological processes, such as cell adhesion, cell activation and chemotaxis, cell growth and differentiation, cell cycle and apoptosis. Based on its extensive biological functions, galectin-3 is considered to be related to cancer, inflammation and fibrosis, heart disease, stroke, etc. Galectin-3 expression levels are associated with various types of fibrosis. Galectin-3 expression is upregulated in liver fibrosis, kidney fibrosis, and idiopathic pulmonary fibrosis (IPF). In acute decompensated heart failure and chronic heart failure populations, high expression of galectin-3 leads to a higher risk of death. Meanwhile, galectin-3 is thought to play an important role in tumor metastasis.

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SKU: 11977133429

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Angela naylor
Bozeman, US
★★★★★ 5
Great product
Size: 3 INCH, Number of Items: 20
Great value sublimates wonderfully! Will buy more!
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Reviewed in the United States on October 26, 2025
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Ash
Belleville, US
★★★★★ 5
Perfect sublimation items!
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These are the cutest items! The print beautifully and easy to open! Will purchase again!!
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Reviewed in the United States on August 19, 2025
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Dan and Danielle Bugay
Fort Morgan, US
★★★★★ 5
Awesome product at a fair price
Size: 8 INCH, Number of Items: 20
We sold out on the first batch we ordered for our first craft show with this product. Works as intended.
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Reviewed in the United States on May 26, 2025
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Tiffany
Charlottesville, US
★★★★★ 5
Great purchase
Size: 8 INCH, Number of Items: 20
Have ordered these wind spinners for years and love them every time! They sublimate beautifully and are each individually wrapped
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Reviewed in the United States on May 30, 2026
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Roberta
Carnegie, US
★★★★★ 4
Not quite 100% happy
Size: 8 INCH, Number of Items: 20
The ones I received made beautiful wind spinners. My only problem with them is that the peel quite a bit so a lot of them I can't even use. :( If the peeling can be fixed, these are fantastic as the colors are beautiful on them.
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Reviewed in the United States on May 2, 2026

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