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Description
GAPDH Recombinant Mouse mAb (S-4721)Product Specification Host Mouse Antigen GAPDH Synonyms Glyceraldehyde 3 phosphate dehydrogenase GAPD Location Cytoplasm, Cytoskeleton, Nucleus Accession P04406P16858 Clone Number S 4721 Antibody Type Mouse mAb Isotype IgG Application WB, IHC P, ICC Reactivity Hu, Ms, Rb, Rt, Zf, Mk, Bv, Dg, Gt, Ck Positive Sample HeLa, MCF7, MDA MB 231, SK OV 3, PC 3, HEK 293, A549, HepG2, PANC 1, HT 29, Ramos, Jurkat, K562, A431, NIH 3T3, RAW264. 7, EL4, C2C12,
Product Specification
| Host | Mouse |
| Antigen | GAPDH |
| Synonyms | Glyceraldehyde-3-phosphate dehydrogenase GAPD |
| Location | Cytoplasm, Cytoskeleton, Nucleus |
| Accession | P04406、P16858 |
| Clone Number | S-4721 |
| Antibody Type | Mouse mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rb, Rt, Zf, Mk, Bv, Dg, Gt, Ck |
| Positive Sample | HeLa, MCF7, MDA-MB-231, SK-OV-3, PC-3, HEK-293, A549, HepG2, PANC-1, HT-29, Ramos, Jurkat, K562, A431, NIH/3T3, RAW264.7, EL4, C2C12, Neuro-2a, mouse brain, C6, PC-12, rat brain, COS-7, zebrafish, chicken liver, dog liver, bovine liver, goat liver, rabbit liver, pig liver |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:10000-1:200000 | Hu, Ms, Rt, Mk, Zf, Ck, Dg, Bv, Rb, Gt, Pg |
| IHC-P | 1:2500 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ubiquitous and highly conserved multifunctional protein that plays a central role in cellular metabolism, primarily catalyzing the sixth step of glycolysis where it converts glyceraldehyde-3-phosphate into 1,3-bisphosphoglycerate while simultaneously reducing NAD+ to NADH. Beyond its canonical function in energy production, GAPDH exhibits remarkable "moonlighting" activities depending on its subcellular localization, participating in diverse processes such as DNA repair, transcriptional regulation, vesicular transport, and the initiation of apoptosis under stress conditions like oxidative damage or nitrosative stress. Due to its constitutive and relatively stable expression across various tissues and experimental conditions, GAPDH has historically been widely used as a loading control in techniques like Western blotting and RT-PCR, although recent studies caution against this practice without validation since its levels can fluctuate significantly in response to specific metabolic states or pathological conditions. Structurally, it typically functions as a homotetramer, and its involvement in several neurodegenerative diseases, including Huntington's and Alzheimer's, highlights its critical importance in maintaining cellular homeostasis and its potential as a therapeutic target.
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