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Description
Human IL-6 OneStep ELISA KitProduct Specification Antigen IL 6 Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Hu Purification Protein A Stability & Storage 2 to 8 C as supplied. Kit Precision Intra assay: 5. 2% Intra assay: 6. 0% Sample type Cell culture supernatant, Serum, Plasma Assay type Sandwich (quantitative) Sensitivity 0. 14 pg ml Range 2. 1 pg ml 136 pg ml Recovery Cell culture supernatant 90% Serum 98% Plasma 97% Assay time 60 minutes Species
Product Specification
| Antigen | IL-6 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Precision |
Intra-assay: 5.2%； Intra-assay: 6.0% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.14 pg/ml |
| Range | 2.1 pg/ml - 136 pg/ml |
| Recovery | Cell culture supernatant 90% Serum 98% Plasma 97% |
| Assay time | 60 minutes |
| Species reactivity | Human |
| CROSS REACTIVITY | / |
| INTERFERENCE | / |
| NIBSC convert | The dose response curve of the International Standard (21/308) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (21/308) units, use the following equation: NIBSC (21/308) approximate value (IU/mL) = 0.142 x OneStep Human IL-6 value (pg/mL). |
Background
Interleukin 6 (IL-6) is a prototypical cytokine for maintaining homeostasis. When homeostasis is disrupted by infections or tissue injuries, IL-6 is produced immediately and contributes to host defense against such emergent stress through activation of acute-phase and immune responses. However, dysregulated excessive and persistent synthesis of IL-6 has a pathological effect on, respectively, acute systemic inflammatory response syndrome and chronic immune-mediated diseases.
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