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Description
Human MMP-9 ELISA KitProduct Specification Species Reactivity Human Theory This kit adopts double antibody sandwich enzyme linked immunosorbent detection technology. Specific anti human MMP 9 antibodies were pre coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the MMP 9 present in the sample binds to the solid phase antibody
Product Specification
| Species Reactivity | Human | |||||||||||||||||||||||||||
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human MMP-9 antibodies were pre-coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the MMP-9 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm). | |||||||||||||||||||||||||||
| Synonym | 92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, EC 3.4.24, EC 3.4.24.35, Gelatinase B, GELB, macrophage gelatinase, MANDP2, matrix metallopeptidase 9, matrix metalloproteinase 9, matrix metalloproteinase-9, MMP9, MMP-9, type V collagenase | |||||||||||||||||||||||||||
| Composition |
Please use within the expiration date of the kit
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| Background | Matrix metalloproteinases (MMPs), also known as matrixins, are members of the zinc-calcium-dependent proteolytic enzyme family, which can break down the extracellular matrix (ECM) and participate in the processing of various molecules in different subcellular environments. MMPs play important functions in numerous physiological processes, such as embryonic development, morphogenesis, reproduction, and tissue remodeling. Also involved in inflammation as well as autoimmune diseases such as arthritis, cancer and cardiovascular diseases. The amount of newly synthesized MMPs is mainly regulated at the transcriptional level, and the proteolytic activity of already existing MMPs is controlled not only by the activity of the zymogen, but also by the inhibition of enzyme activity by endogenous inhibitors, such as α2-macroglobulin, and tissue inhibitors of metalloproteinases (TIMPs). MMP-9 (also known as gelatinase B, 92 kDa type IV collagenase, 92 kDa gelatinase, type V collagenase) is secreted as a proglycosylation enzyme. Activation of the zymogen involves proteolytic removal of the N-terminal precursor region, resulting in the formation of an active enzyme of 82 kDa. Active human MMP-9 has 72% and 74% amino acid sequence homology with mouse and rat MMP-9, respectively. In addition to the zinc binding site, the catalytic domain contains three consecutive fibronectin type II homologous units that bind to gelatin. A proline-rich hinge region links the catalytic domain to the C-terminal heme-like domain. In vitro experiments, after treatment with 4-aminophenyl ethyl acetate (APMA), the enzymogen can not only produce active enzyme, but also produce a C-terminal truncated form with equivalent activity. MMP-9 can degrade components in ECM, especially with very high specificity for denatured collagen (gelatin). MMP-9 also cleaves collagen types III, IV, V, XI, as well as elastin, nestin-1, and vitronectin. MMP-9 can also cleave a variety of chemokines and growth factors (e.g., IL-1β, CXCL8/IL-8, CXCL7, CXCL4, CXCL1, LatentTGF-β, membrane-bound TNF-α, VEGF, and FGFbasic), beta-amyloid, substance P, myelin basic protein. This action may increase or decrease the biological activity of these soluble factors, and may also cause them to be released from association with the ECM. MMP-9 can also trigger signaling pathways through various membrane proteins or induce them to fall off the cell membrane to inhibit signaling pathways (E.g. CD44, E-cadherin, integrin, ICAM-1 and IL-2Rα). MMP-9 is produced by a variety of normal and transformed cells, including neutrophils, monocytes, macrophages, astrocytes, fibroblasts, osteoclasts, chondrocytes, keratinocytes, endothelial cells, and epithelial cells. It affects physiological and pathological angiogenesis and vascular remodeling. Activated neutrophils release the MMP-9 precursor and do not bind to TIMP-1, allowing pro-angiogenic FGF-2 to be released from the ECM. The complex of MMP-9 and TIMP-1 failed to induce the release of FGF-2. Neutrophil-derived MMP-9 can exacerbate the inflammatory response, inducing the release of additional neutrophil MMP-9 by the production of collagen-derived polypeptides. MMP-9 also plays an important role in bone formation and remodeling, methamphetamine-induced behavioral sensitization and response, regulation of neuronal synaptic remodeling, trophoblast invasion during implantation, and activation of serine protease inhibitor α1. MMP-9-mediated shedding of adhesion molecules also has a direct effect on tumor cell invasion. Circulating levels of MMP-9 are elevated in many inflammatory disorders, including the formation of intraluminal thrombus, atherosclerosis, Crohn's disease, hepatitis C virus infection, colorectal cancer, and Duchenne muscular dystrophy. The ratio of MMP-9 to TIMP-1 was elevated in multiple sclerosis sera and cystic fibrous sputum but decreased in cytomegalovirus-infected sera. The level of free MMP-9 and the level of MMP-9 and lipocalin-2/NGAL complex were elevated in the urine of patients with ovarian cancer and patients with uterine urethral tract infection, respectively. |
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| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. 6. For scientific research only, not for in vitro diagnosis. |
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| Storage Temp. | Kit unopened, stored at 2-8 °C. | |||||||||||||||||||||||||||
| Test Range | 31.3pg/mL-2000pg/mL |
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4.0 ★★★★★
Based on 20 reviews
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Product Reviews
★★★★★ 5
Strong Omegaverse Comfort and a Attention Grabbing Plot
Format: Kindle
Jillian West never misses when it comes to Omegaverse, and Not Ready is no exception. This story was the perfect blend of cozy comfort and emotional depth while still delivering a strong plot.
Vale is such a powerful heroine, she is strong, capable, and determined but I love that she still allows her pack to love and take care of her. It’s that balance of independence and vulnerability that makes her so relatable.
The relationship dynamics were amazing: Bishop is steadfast and completely head over heels, Mercy is skeptical but protective in his own way, and Holt is the hesitant one whose slow fall is so satisfying to watch unfold.
The romance hits that sweet spot between insta-love and cautious build, keeping me hooked the entire way through. And that ending. Oh my god, the cliffhanger! I need the next book in this duet immediately.
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Reviewed in the United States on August 28, 2025
★★★★★ 5
Interesting
Format: Kindle
So I will say I enjoyed the story, for sure had its moments where it dragged but it was a great story. I really liked that omegas picked their alphas/make the pack. Normally the Alphas make it and the omega fits in with them which is great but I enjoyed this new version where all the power basically went to the omega. It was a nice change of pace. I can admit some of the weird bedroom stuff with her being pregnant was odd, it’s really not hard to do stuff when pregnant (I know I’ve had two and it’s normal and even encouraged at the end especially if you want the baby out). But I like the story as a whole and will read the second, I do hope the next one isn’t dragged bc it stopped being action or tense after she met her alphas and I don’t think it was brought up or properly done when they tried to do it. More sweet after she left.
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Reviewed in the United States on November 11, 2024
★★★★★ 3
I’m a little disappointed.
Format: Kindle
I usually like Jillian West’s books but this one was missing a lot for me. The pregnancy didn’t come across as real. She’s on her feet for 12 hour days but is perfectly healthy at 8 months pregnant? Yet the week she moves in all of a sudden she’s not? She is planning on actually running during one of the plot buildups. But at 8 months pregnant that’s incredibly hard to do. The lack of breathing ability and lung space, the change in body center, mass, and gravity. All of it prohibits running, unless you’re an athlete this didn’t come off as at all realistic.
I didn’t feel any connection with the alphas. There wasn’t any emotional connection. It could be because of the tense it was written in. But I didn’t get any deep feelings out of this. It came across as checking off boxes. Even the spicy scenes weren’t really believable for me. I wanted to see them fall for her, and it just kind of all fizzled. Even Bishop.
One thing I did really like was the ending. I did not see it coming and I’m interested in reading book two because of it. But on the whole this book was mostly disappointing for me.
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Reviewed in the United States on March 16, 2024
★★★★★ 4
4.25 stars
Format: Kindle
Vale is an 8 month pregnant omega working as a waitress at a strip club and a cam girl. She starts to get very creepy vibes from a regular at the club, and her baby daddy ghosted her. She has had an online relationship with a man named Bishop through her cam girl status. One night, bishop was paying to watch her sleep and ansthe creepy regular Andrew break in and watch her sleep he tells vale to come to him at his business now. She flees and finds herself at a large security company with some.hot of alphas who are there to help her. This imegaverse is a little different than I have read, but I am thoroughly enjoying it. Vale is not a traditional omega she was raised by a single beta mom, and the alphas are not normal alphas they have never really loved pack life. But they are ruthless mercenaries. They need her, and she needs them. I love the aspect of the stalker and now the plot twists at the end, so so good. Sometimes, it seemed a little slow and stale mated, but since this a duet, I think It was just her starting to have Vale get to know her alpha suitors. Cliffhanger for sure with this one.
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Reviewed in the United States on September 9, 2024
★★★★★ 5
That ending 😫
Format: Kindle
I fell into a false sense of security and really thought this was gearing towards a happy ending. Then I realized there’s no work they don’t punish Andrew. I really liked Vale’s character. I don’t normally read books with pregnancy but going into this knowing she was pregnant made it more enjoyable for me.
I loved Bishops devotion to her and her happiness. I also loved that Holt and Mercy couldn’t fight their attraction to her. I love scent matches so very much. I’m so curious to see how this duet will end up. And I need to pay more attention and notice that a book I’m starting is a duet to begin with lol
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Reviewed in the United States on February 21, 2025