Mouse IgE ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti mouse IgE capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IgE in the sample binds to the solid-phase antibody. After thorough washing to remove unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After thorough washing again, TMB chromogenic substrate was added to avoid light for color development. The depth of color reaction has a positive correlation with the concentration of IgE in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value under the condition of 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, biotin labeled IgE detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 1.10 ng/ml

Detection Range: 15.63-1000 ng/ml

Recovery Range: 89-104%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Immunoglobulin E (IGE)They are a class of antibodies that exist only in mammals. The main function of IgE is to produce immunity to parasites, such as worms, Schistosoma mansoni, Trichinella spiralis and Fasciola hepatica. IgE plays a key role in type I hypersensitivity reactions, such as allergic asthma, most sinusitis, allergic rhinitis, food allergy, chronic urticaria and atopic dermatitis. IgE also plays an important role in the response to allergens, such as allergens, bee stings, antigens used in desensitization immunotherapy. Although IgE content is the least (IgE content in normal human serum only accounts for 0.05% of all Ig classes), it can trigger the strongest inflammatory response. The content of IgE in the blood of patients with hereditary allergy is 10 times higher than that of normal people. In a variety of autoimmune diseases, such as systemic lupus erythematosus (SLE), rheumatoid arthritis (RA) and psoriasis, the level of IgE has increased. It is presumed that IgE plays an important pathogenic role in RA and SLE by inducing hypersensitivity.