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Description
Phospho-PKA Substrate (RRXST) Recombinant Rabbit mAb (S-2623-148)Product Specification Host Rabbit Antigen Phospho PKA Substrate (RRXST) Immunogen Synthetic Peptide Clone Number S 2623 148 Antibody Type Recombinant mAb Isotype IgG Application ELISA, WB Reactivity Hu, Ms Positive Sample starve overnight and Calyculin A treated HeLa, Calyculin A treated NIH 3T3 Purification Protein A Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05% BSA, 0. 03%
Product Specification
| Host | Rabbit |
| Antigen | Phospho-PKA Substrate (RRXST) |
| Immunogen | Synthetic Peptide |
| Clone Number | S-2623-148 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ELISA, WB |
| Reactivity | Hu, Ms |
| Positive Sample | starve overnight and Calyculin A treated HeLa, Calyculin A treated NIH/3T3 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms |
Background
Phospho-PKA Substrate (RRXST) is a recognition motif for substrates specifically phosphorylated by protein kinase A (PKA). Within this motif, serine (S) or threonine (T) serves as the phosphorylation site, flanked on its N-terminal side by two arginine residues (RR). The position immediately following the second arginine can be any amino acid (X). This highly conserved pattern of basic amino acids ensures that PKA can specifically recognize and catalyze the phosphorylation of serine/threonine residues in its substrates, thereby regulating various critical cellular signaling pathways, including glycogen metabolism, gene transcription, and cell proliferation. Specific phospho-antibodies targeting this motif have become essential research tools for detecting PKA activity and its downstream signaling events.
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