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Description
mIHC Dye (TSA770) Two-step MethodProduct Specification References 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008. 9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79 80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46 58. 4.
Product Specification
| References | 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. 4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 770 component A, TSA770 dye component B, signal amplification reaction solution |
| General Notes | 1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged. 2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained. 3. The tissue should contain at least more than 1000 cells. 4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect. 5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h. 6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation. 7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper. 8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h). |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments. |
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★★★★★ 5
Great Wall Shelves – Sturdy, Easy to Install, and a Fantastic Value!
Size: 4 pcs: 15.7*5.7, Color: White
I’m really happy with these wall shelves! They’re sturdy, well-built, and hold up perfectly under weight—no concerns about them sagging or bending. The installation was super simple, and everything was clearly labeled with no missing parts, which made the whole process smooth and stress-free.
I love that they come in a set of four, making them perfect for displaying books, plants, or decorative items in multiple spots around the room. The design is sleek and minimal, so they fit in with pretty much any style of decor.
Overall, these shelves are a great value, and I would definitely purchase them again. Highly recommend if you’re looking for reliable and easy-to-install shelving!
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Reviewed in the United States on November 7, 2024
★★★★★ 4
Looks good but don’t ever take down.
Size: 4 pcs: 15.7*5.7, Color: White
Be ready for gargantuan holes to be put in your wall.
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Reviewed in the United States on March 29, 2026
★★★★★ 5
Great purchase
Size: 4 pcs: 15.7*5.7, Color: White
Easy to install. Great quality. Would buy it again.
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Reviewed in the United States on March 20, 2026
★★★★★ 5
Beautiful and very easy to install
Size: 4 pcs: 15.7*5.7, Color: White
They look great and very easy to install, I really recommend this product.
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Reviewed in the United States on May 28, 2026
★★★★★ 3
Just Ok.
Size: 4 pcs: 15.7*5.7, Color: Maple Wood, Size: 4 pcs: 15.7*5.7, Color: Maple Wood
They look nice but they aren’t sturdy and do not hold much weight. I’ve bought better ones on Amazon.
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Reviewed in the United States on January 12, 2026
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